IonPac CS14 Manual
Doc. No. 034848-10
Page 26 of 54
5.10 Elution of Mono-, Di- and Triethylamines With Resolution Enhancement Through the
Addition of Solvent to the Eluent
The determination of mono-, di- and triethylamines in the presence of ammonia, alkali and alkaline earth metals can be
accomplished by isocratic elution using 10 mM methanesulfonic acid (MSA). Reductions in runtimes can be accomplished by
the addition of a small amount of solvent to the eluent in combination with a step gradient program.
Sample Loop Volume:
4.5 µL (2-mm), 18 µL (4-mm)
Analytical Column:
IonPac CS14 Analytical Column
Eluent:
See Chromatogram
Eluent Flow Rate:
0.25 mL/min (2-mm), 1.0 mL/min (4-mm)
SRS Suppressor:
Cation Self-Regenerating Suppressor, CSRS ULTRA (2-mm or 4-mm)
Recycle Mode or External Water Mode
or MMS Suppressor:
Cation MicroMembrane Suppressor, CMMS III (2-mm or 4-mm)
MMS Regenerant:
100 mN TBAOH
(tetrabutylammonium hydroxide)
Expected Background Conductivity:
< 1 µS
Storage Solution:
Eluent
Eluent 1:
Type I DI Water
Eluent Flow Rate:
1.0 mL/min
Eluent 2:
100 mM MSA
(4-mm System)
Eluent 3:
10% Acetonitrile
TIME (min)
%E1
%E2
%E3
Comments
Equilibration
0.0
86
9
5
Equilibrate to 5.0 mM MSA / 2.5% ACN
7.0
86
9
5
Analysis
0.0
86
9
5
Inject, 5.0 mM MSA / 2.5% ACN
0.1
86
9
5
Inject Valve to Load Position
6.0
74
11
15
11 mM MSA / 1.5 ACN
13.0
74
11
15
11 mM MSA / 1.5 ACN
