Appendix A
– Current Settings
Thermo Scientific
Product Manual for the ERS 500 Suppressor
Page 76 of 84
031956-11
For Research Use Only. Not for Use in Diagnostic Procedures.
Table 16
Optimum Current Settings for the Dionex AERS 500 Carbonate in the
AutoSuppression Recycle and External Water Modes
Column
Eluent
Eluent Flow Rate (mL/min)
(2 mm)
(4 mm)
Optimum Current (mA)
(2 mm)
(4 mm)
AS4A-SC
1.8 mM CO
3
2-
/ 1.7 mM HCO
3
-
0.5
2.0
9
34
AS9-HC
9.0 mM CO
3
2-
0.25
1.0
15
58
AS12A
2.7 mM CO
3
2-
/ 0.3 mM HCO
3
-
0.38
1.5
7
28
AS14
3.5 mM CO
3
2-
/ 1.0 mM HCO
3
-
0.3
1.2
8
31
AS14A
8.0 mM CO
3
2-
/ 1.0 mM HCO
3
-
0.5
(1)
1.0
28
55
AS22
4.5 mM CO
3
2-
/ 1.4 mM HCO
3
-
0.3
1.2
11
41
AS22-Fast 4.5 mM CO
3
2-
/ 1.4 mM HCO
3
-
0.5
2.0
17
67
AS23
4.5 mM CO
3
2-
/ 0.8 mM HCO
3
-
0.25
1.0
8
32
(1)
3 mm format
The Dionex AERS 500 cannot be used with legacy systems that only provide discrete (50, 100,
300 and 500 mA) current settings.
Table 17
External Water Flow Rates for Dionex AERS 500 Carbonate (4 and 2 mm)
Dionex AERS 300
Format
Regenerant Flow Rate
(mL/min)
(1)
4 mm
3 – 5 mL/min; at least 2x the eluent flow rate
2 mm
1 – 2 mL/min; at least 2x the eluent flow rate
(1)
Measured with power ON using a graduated cylinder
A.3. Optimum Current Settings for Common Eluents; Dionex CERS 500 (4 mm and
2 mm) and Dionex CERS 500e (4 mm and 2 mm)
The Dionex Cation Electrolytically Regenerated Suppressor (Dionex CERS 500 and Dionex
CERS 500e) uses water as the regenerant and has the ability to provide continuous suppression.
Table 18 - 20 list the eluent concentrations and flow rates of commonly used mobile phases
used in cation separations and the optimum / recommended current level to suppress the eluent.
The operation of the Dionex CERS 500 and Dionex CERS 500e requires a constant flow of
water over the electrodes in a direction that is countercurrent to the flow of the eluent.
In the AutoSuppression Recycle Mode, the eluent leaving the conductivity cell is recycled
through the regenerant chambers as the water supply. This eliminates the need for an external
regenerant water supply and delivery system. When the Dionex CERS 500 is operating in this
mode, the amount of water flowing through the regenerant chambers is limited to the eluent
flow rate. The AutoSuppression Recycle Mode cannot be used with eluents containing any
organic solvents.
The AutoSuppression External Water Mode requires an external source of deionized water for
the regenerant chambers. When the Dionex CERS 500 is operating in this mode, the amount of
water flowing through the regenerant chambers is independent of the eluent flow rate. Because
of this, higher regenerant flow rates are achievable, see Table 20. Higher regenerant flow rates
translate in improved signal-to-noise ratios compared to the AutoSuppression Recycle Mode.
The AutoSuppression External Water Mode is the mode used if organic solvents (up to 40%) are
present in the eluent. It eliminates the potential for buildup of contaminating ions resulting from
the oxidation of solvents.
