Techne TC-3000G, Руководство оператора

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PROGRAMMING OPTIONS
There are two options available for creating programs on the TC-412:
1. Copy and edit one of the pre-written templates.
2. Create a new program from the defaults.
OPTION 1: CREATING PROGRAMS USING TEMPLATES
There are two pre-written basic PCR templates: a two-step and a three-step template. These can be
copied and modified as required.
Other templates available include Ice bucket (incubation at 4ºC), Ligation (incubation at 15ºC) and an RT-
PCR template.
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Use the "Down Arrow" key to select an
existing 'Template' program from the menu,
e.g. '2 Step Template' or '3 Step Template'.
Press the "Enter" key to accept it.
The template is already defined and can be run
as it is or easily copied, modified and saved
under a new name.
Use the "Down Arrow" key to select 'Copy
Program'
Press the "Enter" key to accept it.
2 STEP TEMPLATE [501]
Run program
View program
[ , to select ]
2 STEP TEMPLATE
Heated Lid 105°C
Preheat Lid on
Pause off
-------------------------
In denat 94°C 5m00
Hot Start off
------- Stage 1---------
Number cycles 30
Step 94.0°C 0m30 max
Step 68.0°C 1m00 max
-------------------------
Final Extn 72.0°C 5m00
Final hold 10.0°C
3 STEP TEMPLATE
Heated Lid 105°C
Preheat Lid on
Pause off
-------------------------
In denat 94.°C 5m00
Hot Start off
------- Stage 1---------
Number cycles 30
Step 94.0°C 0m30 max
Step 55.0°C 0m30 max
Step 72.0°C 0m30 max
-------------------------
Final Extn 72.0°C 5m00
Final hold 10.0°C
Run program
View program
Copy program
[ , to select ]
* * * New Program * * *
2 STEP TEMPLATE 501
3 STEP TEMPLATE 502
[ , to select ]
TC412 Techne Manual 19/12/08 3:38 PM Page 28