Techne TC-3000G, Руководство оператора

Страница: 32 / 51

EXAMPLE PROGRAMS
A couple of example thermal cycling programs are shown below.
Typical 3-Step PCR 3-Step PCR with multiple 'Stages'
31
PROGRAMMING
To add a stage and then edit it
Heated Lid 105°C
Preheat Lid on
Pause off
-------------------------
In denat 94°C 5min
Hot Start off
-------- Stage 1 --------
Number cycles 30
Step 94.0°C 0m30 max
Step 55.0°C 0m30 max
Step 72.0°C 0m30 max
-------------------------
Final Extn 72.0°C 5m
Final hold 4°C 10m
Heated Lid 105°C
Preheat Lid on
Pause off
-------------------------
In denat 94°C 5min
Hot Start off
-------- Stage 1 --------
Number cycles 10
Step 94.0°C 0m30 max
Step 60.0°C 0m30 max
Step 72.0°C 0m30 max
-------- Stage 2 --------
Number cycles 20
Step 94.0°C 0m30 max
Step 55.0°C 0m30 max
Step 72.0°C 0m30 max
-------------------------
Final Extn 72.0°C 5m
Final hold 4°C 10m
Create a new program by selecting ** New
Program ** from the menu and press
"Enter"
Give the program a name and edit any of the
default fields as described above.
Use the "Down Arrow" key to scroll to
the dotted line below 'Hot Start'; it will start
to flash.
Press "Enter" to insert a stage.
* * New Program * * [ ]
2 STEP TEMPLATE 501
3 STEP TEMPLATE 502
[ , to select ]
UNNAMED #
Heated Lid 105°C
Preheat Lid on
[ , to edit ]
In denat 94.0°C 5m00
Hot Start off
-------------------------
[ to insert stage ]
[ to save ]
TC412 Techne Manual 19/12/08 3:38 PM Page 33