Geode H14000 User Manual
20
iii.
Release
The release phase consists of the return of temperature and pressure conditions to the ambient
state. It should always be the last step of Crystal Digital PCR™ of the Geode program.
Figure 7: Typical graph showing the evolution of the measured pressure and temperature
during the run of a default program for Sapphire chips
loaded on the Geode named “Template
Stilla PCR 45 cycles”.
1) In the main panel on the screen of the Geode
, press “Run”
, then press the
“Open icon”
.
In the “Open program” window.
2) Select the cycling
program in the “local/scripts” or “/templates” sub-folder.
3) Then, press the
“Open icon”
and the
“play icon”
to start the program.
4) Visualize the graphical progression of the partitioning and thermal cycling programs by
pressing on
“Live Logs”
accessible from the main panel.
5) Press
on “Run”
to go back to the remaining time estimation.
Note: The remaining time of a program is indicative and relies on the effective temperature ramp
rate of the thermal plate and the duration of the stabilization phase at each step. Thus, it could
be slightly overestimated by the Geode.
When the Crystal Digital PCR
™ has ended, a pop-up window is displayed on the Geode screen
“PCR successfully completed, touch the screen to continue”. Touch the Geode screen and open
the Geode lid.
6) Remove the chips from the respective magnetic frame and close the Geode lid.
a. Hold the Sapphire chips by the tall white caps.
b. Hold the Opal chips by the bridge in the middle of the Opal chip PCR caps.
