Geode H14000 User Manual
16
Crystal Digital PCR™ Geode protocol
There are two microfluidic consumables compatible with the Geode: the Sapphire chips and the
Opal chips.
The Sapphire chips are compatible with all Geode versions.
The Opal chips are only compatible with Geode version H14000 running with software version
2.6.0 and above. Geode versions H13000 and below are not compatible.
For detailed instruction for loading the samples in the Sapphire and Opal chips, please refer to
the respective Instructions for Use and the Quick User Guides available here:
https://www.stillatechnologies.com/technical-resources/naica-system-prism3/
.
Loading
chips for Crystal Digital PCR™ on the Geode
1) Turn on the power switch located at the back panel of the (
Fig. 3 B
) and wait for initialization.
Figure 3: Front (A) and back (B) view of the Geode. USB ports are optional and intended for
data import or export; the ethernet port is optional.
2) Check that the pressure source is connected to the pressure inlet behind the device (
Fig. 3B
).
The pressure tube coming from the pressure source (compressed air system (ISPTLAPG3)) can
be connected to the instrument by simply pushing it in the pressure inlet until it does not go any
further.
3) Switch on the pressure source, wait for a minute, and ensure that the pressure delivered to
the Geode is between 1,250 ± 50 mbars (check the screen of the device or the screen of the
pressure controller behind the device to ensure the accuracy of the pressure delivered to the
Geode) (
Fig. 3B
). Although the input pressure is adjusted at installation, it can be adjusted
manually but should not exceed 1300 mbars.
4) Open the lid of the Geode by pulling the black handle downward (
Fig. 6
). Check that the
thermal plate is empty and cleaned. Make sure the gasket around the thermal plate of the Geode
is in good working order, and that the thermal plate is kept free of dust particles or moisture.
5) At installation, the Sapphire chip adaptors and the Sapphire chip magnetic frame are in place
by default. To change the Geode configuration to Opal chip operation, place the provided
magnetic adapters next to the existing clamps and make sure they are secured (
Fig. 4
).
