STEMCELL RoboSep 20000, Техническое руководство

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THIS PRODUCT IS MANUFACTURED AND CONTROLLED UNDER A QUALITY MANAGEMENT SYSTEM CERTIFIED TO ISO 13485 MEDICAL DEVICE STANDARDS.
FOR RESEARCH USE ONLY. NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES.
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1
Pour off supernatant.
Positive Selection: Desired,
labeled cells remain in the
tube.
Negative Selection: Desired
unlabeled cells are in the
new tube.
Place tube
in magnet
Incubate
Incubate
Add EasySep™
selection cocktail
Cell suspension
1
2
3
Add EasySep™
magnetic particles
1 INTRODUCTION
RoboSep™ (Catalog #20000) is a laboratory instrument that automates EasySep™ immunomagnetic cell separation. EasySep™ is a
column-free magnetic cell separation technology that enables both positive and negative selection of desired cells from virtually any
species. Using an integrated color touch screen, RoboSep™ can be programmed to purify cells from up to four samples at once. The
user simply loads the consumables as indicated on the built-in touchscreen and starts the experiment; RoboSep™ then automatically
executes the EasySep™ immunomagnetic labeling and magnetic separation steps using disposable pipette tips.
RoboSep™ is intended for research applications only. Please make sure that you have read page ii of this manual before using the
instrument.
1.1 SYSTEM DESCRIPTION
1.1.1 EasySep™ Magnetic Cell Separation Technology
EasySep™ is a powerful immunomagnetic cell selection procedure that
combines the specificity of monoclonal antibodies with the simplicity of a column-
free magnetic system. EasySep™ can be used as either a positive or a negative
selection approach to purify your cells of interest.
With EasySep ™ positive selection , highly purified cells are obtained by
targeting the cells of interest with antibodies directed against one or more
specific cell surface antigens. These targeted cells are cross-linked to
EasySep™ magnetic particles. The sample tube containing the cells is then
placed directly in a specially designed EasySep™ magnet. The unique magnet
generates a high gradient magnetic field in the interior cavity that is strong
enough to separate cells labeled with EasySep™ magnetic particles without the
additional magnetic field gradients provided by a column matrix. The cells that
are not bound to the magnetic particles are removed either by pipetting off
(RoboSep™) or by inversion of the magnet (manual EasySep™). To obtain a
highly purified cell population, the cells remaining in the tube can undergo
additional separations prior to being collected by removing the tube from the
magnet (Figure 1). Unlike larger particles used with other column-free systems,
the EasySep™ magnetic particles used in positive selection procedures do not
interfere with subsequent flow cytometric analysis and do not need to be
removed.
For EasySep™ negative selection applications, unwanted cells are targeted for
depletion using a cocktail of monoclonal antibodies directed against specific cell
surface antigens. The labeled unwanted cells are then cross-linked to EasySep™
magnetic particles. The sample tube containing the cells is placed in the specially
designed EasySep™ magnet. The desired (unlabeled) cells are removed either
by pipetting off (RoboSep™) or by inversion of the magnet (manual EasySep™).
Figure 1- RoboSep™ fully automates the EasySep™ procedure for
positive (illustrated below) or negative selection.