STEMCELL RoboSep 20000, Техническое руководство

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Transport: This command is used to transport fluids from one vial or
tube to another (Figure 28). A label is required to describe the steps. The
source and destinations need to be appropriately specified, as well as
whether a volume relative to the sample volume or an absolute volume is
added (see “Volume,” above). The default tip rack is in Quadrant 1, but a
tip rack in another quadrant can be specified by activating the Tip Rack
check box. Free-Air Dispense can also be selected. For negative
selection protocols using D magnetic particles or Streptavidin
RapidSpheres™, the free-air dispense should be selected when
transporting from the sample tube to the selection tube. For all other
transports from the sample tube to the selection tube, the free-air
dispense should not be selected.
Mix: This command is used to mix the contents of a vial or tube. A label
is required to describe the step (Figure 29). The source is the tube
containing the sample or reagent to be mixed, and there is an option to
specify the extension time (see Section 3.3.3) and the tip rack to be
used. There are tip-use rules which decide which tip will be used for
each mixing step; these rules are automatically applied by RoboSep™.
Selecting “Home” prompts RoboSep™ to perform the “Home All”
command (see below) prior to the “Mix” step; this option is not necessary
for most RoboSep™ applications. If Relative Proportion is selected, a
fraction of the calculated volume in the vial will be mixed. Most standard
RoboSep™ mix steps use a relative volume of 0.33. If Absolute Volume
is selected, the set volume (entered by the user) will be mixed. The gap
between the mixing tip and the bottom of the tube (typically 100 µL)
should also be entered. The number of mix cycles can be modified from
1 to 5; most standard RoboSep™ mix steps iterate 3 times.
Incubate: This command allows for incubation of the sample with
reagents prior to another step in the sequence being performed
(Figure 30). A label is required to describe the step. As before, there are
options for extension time and home.
Separate: This command is functionally the same as the “Incubate”
command and is used to set incubation times for magnetic separations
(Figure 30). The separation time is the minimum amount of time before
the next command in the sequence is executed. Typical values for
separation time are between 150 and 900 seconds (2.5 and 15 minutes).
Figure 30 – Incubate or separate command
Figure 28 – Transport command
Figure 29 – Mix command