16
STEINDORFF
®
4.1.
Reflected Light Brightfield/Darkfield Observation
o
The following flow shows the basic operating procedure for reflected light brightfield or darkfield observation.
Controls Used
Page
Select the brightfield (BF) or darkfield (DF) observation
Mirror selector lever
Main switch
ND filter knob
Light path selector knob
Stage plate
Revolving nosepiece
Coarse/fine adjustment knobs
Brightness adjustment knob
Binocular tube
Diopter adjustment ring
AS knob
FS knob
Revolving nosepiece
Coarse/fine adjustment knobs
Filter insertion slot
Brightness adjustment knob
(P. 13)
(P. 9)
(P. 12)
(P. 13)
(P. 10)
(P. 11)
(P. 9)
(P. 10)
(P. 10)
(P. 14)
(P. 13)
(P. 12)
(P. 9)
Start observation.
Adjust the brightness.
Insert the required filters
Engage the desired objective in the light path and bring the
specimen in focus.
Adjust the aperture iris diaphragm and field iris diaphragm.
o
Open both iris diaphragms in case of DF observation.
Adjust the interpupillary distance.
Adjust the diopter.
Adjust the brightness.
Bring the specimen in focus.
Engage the 10X objective in the light path.
Place the specimen on the stage.
Select the light path
Check interlocking of the ND filter
Disengage the analyzer, polarizer, filter, etc.
from the light path.
Set the main switch to “I” (ON).
4.
OBSERVATION METHODS
