13
STEINDORFF
®
Fig. 27
Fig. 28
②
④
①
③
⑤
Field iris diaphragm image
Field of view of eyepiece
3.10.
Selecting the Light Path of the trinocular Tube
1.
Slide the mirror selector lever
①
toward the indication of
the mirror for the desired observation method.
BF: Reflected light brightfield observation
DF: Reflected light darkfield observation
o
Be sure to slide the mirror selector lever until it contacts the
stopper.
3.11.
Centering the Field Iris Diaphragm (FS)
1.
Slide the mirror selector lever
①
to “BF”.
2.
Engage the 10X objective by rotating the revolving
nosepiece, place the specimen on the stage and adjust
approximate focusing.
3.
Pull out the FS knob
④
on the reflected light illuminator to
reduce the aperture iris diaphragm a little.
4.
Rotate the two FS centering screws
②
using the Allen
screwdriver to adjust so that the field iris image becomes
concentric with the field of view.
5.
While pushing in the FS knob
④
, open the field iris
diaphragm until the field iris image inscribes the field of
view. If the image is found to be eccentric, adjust the
centering again.
6.
Open the field iris so that its image is almost the same size as
(Fig. 28 subscribes) the field of view.
Using the Field Iris Diaphragm
In reflected light brightfield observation
The field iris diaphragm adjusts the illuminated area to obtain
an image with high contrast. According to the objective in
use, adjust the FS knob
④
of the reflected light illuminator
until the iris image circumscribes the field of view to block
unnecessary light.
In reflected light darkfield observation
The field iris diaphragm must be opened by pushing in the
FS knob.
