Shel lab BACTRON300, Инструкция по установке и эксплуатации, Страница: 62 / 86

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USER MAINTENANCE (CONTINUED)
L
EAK
C
HECK
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E
MPTY
U
NIT
Use this comprehensive procedure to verify the atmospheric integrity of the workspace chamber
when the BACTRON can be taken out of operation. All samples should be removed from the
workstation prior to carrying out this procedure as aerobic atmosphere will be present in the
chamber.
This procedure places the unit at a steady state temperature and atmospheric pressure, then
carries out a set of leak checks.
1. Turn off the BACTRON.
2. Remove the left arm port sleeve and open the left arm port door.
3. Remove the catalyst cartridge from the BACTRON.
a. The catalytic production of water vapor reduces the volume of atmosphere in the
chamber and may interfere with performing an accurate leak check.
4. Verify the manometer is filled with water up to the top reference ring (the fill line) while the
BACTRON is at room atmospheric pressure with the arm port open.
5. Check the integrity of the pass box door gaskets.
a. Replace if brittleness, dryness, or cracks are present.
b. Clean the gaskets with warm water and soap if sticky or dirty. Dry and seat securely on
the pass box mounting frames.
6. Close and latch both pass box doors.
a. Verify that window panels of both doors sit flush against the gaskets.
7. Check the arm port door ring seals for signs of damage or wear.
8. Close and secure the left arm port.
9. Check that both the arm port doors are secure, with the locking bars in the horizontal
position, and the knobs snugly tightened clockwise using wrist strength.
10. Check that the AMG gas regulator is set to 15 to 20 psi.
a. Open the gas cylinder valve all the way on if not already opened.
11. Turn on the BACTRON.
a. Check that the Chamber Gas light illuminates. Listen for the accompanying clicking
noise of the chamber gas solenoid opening and closing.
b. The BACTRON should begin injecting AMG to establish overpressure in the chamber.
12. Set the temperature of the incubator(s) to 0.
a. An incubator actively heating from room temperature to achieve a set point increases
air pressure in the workstation due to thermal expansion of the chamber atmosphere.
b. This can interfere with performing an accurate leak check.