Shel lab BACTRON300, Инструкция по установке и эксплуатации, Страница: 43 / 86

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OPERATION (CONTINUED)
M
OVING IN THE
P
RESSURIZED
C
HAMBER
There is a minor, but noticeable feeling of resistance while working in the overpressure
environment of the workspace chamber. Moving in the chamber will temporarily increase the
chamber pressure. Large and fast movements can cause the manometer to bubble as anaerobic
atmosphere is forced out of the BACTRON. This results in increased AMG usage as gas injections
are made to maintain overpressure. Move deliberately and steadily when in the chamber.
Withdrawing one sleeved arm partly into the arm port while reaching in with the other (a movement
combination that looks something like swimming) can help reduce pressure and gas consumption.
Use the vacuum foot pedal to reduce any ballooning of the sleeves.
A
NAEROBIC
M
ONITORING
S
TRIPS
In the next procedure (Verifying an Anaerobic Atmosphere) an anaerobic
monitoring strip packet is opened to verify that a strict anaerobic atmosphere has
been established in the chamber.
Strip Indicator Functions

The monitoring strips remain white in the absence of oxygen

The strips turn pink in the presence of oxygen

The more color on the strip, the more oxygen is present.

The strips do not lose color after having been exposed to O
2
. A strip hold its
pink coloring, even if oxygen is removed from the chamber atmosphere.

A new packet must be opened to resume monitoring for O
2
once all oxygen is
purged or scrubbed from the chamber atmosphere.
Opening and Handling
Keep the following points in mind when opening and handling the monitoring strips.

Do not touch the monitoring strip. Doing so risks contaminating the strip and creating false
oxygen positive coloring, even in a fully anaerobic environment.

For the best accuracy and to lengthen the amount of usable time, the strip should be left in
the packet, partly exposed. This avoids contamination from surfaces and wicks oxygen-
detecting fluid from bottom of the packet.

Bending the edges or partly folding the packet allow it to stand upright.

Once a monitoring strip has dried out, it can no longer indicate new oxygen contamination.

There should be one opened and moist anaerobic monitoring strip in the workspace
chamber at all times while maintaining anaerobic conditions. Place the strip where it will be
clearly visible.
Figure 27: Saturated Pink
(Significant O
2
Present)