SEMC TF20, Руководство пользователя, Страница: 5 / 30

Last updated 10/29/2020
4
End of Day Checklist
1. After your screening and/or data collection is done, remove the cryo holder from the microscope
a. See Cryo Holder Insertion/Retraction Guide for F20 for detailed instructions
2.
If you used the DE20, make sure the camera is “retracted” and “warm up”
3. Cryo cycle the microscope
a. column valves closed
b. reset holder
c. flu screen is down
d. objective aperture is out
e. high mag lenses on (send scope to EN/FA/FC preset; anything in the SA mag range)
f.
click “Cryo Cycle” on microscope PC (240 minutes)
4. Remove cold trap from the stand when IGP1 = 99
a. You can keep the remaining LN
2
inside the cold trap (off the coils) for tomorrow’s user
5. Bake out the cryo holder
a. See Dry Pumping Station User Guide (for cryo holders) for detailed instructions
6. Log off Leginon workstation
7. Close Leginon client on both camera and microscope
8. Cleanup the bench space for the next user
9. Log any issues into the EM notebook or notify SEMC staff directly