SEMC TF20, Руководство пользователя, Страница: 11 / 30

Last updated 10/29/2020
10
iii.
Scope = tf20- d394→ “run”
6. Upload your presets
a.
Preset manager → “import presets from another session” (* icon)
i. TEM = tecnai; Digital Camera = DE-20
b.
“Find” presets from past 20 days; select desired preset (usually from the most recent user)
c.
Highlight all the presets (GR, SQ, HL, FA, FC, EN) and “import” → “done”
d. Close window
7.
“Cycle” presets to minimize hysteresis in the beam
a.
Under “Preset Manager” node, select “settings” button and check the “cycle presets”
AND “optimize cycling” option
8. Open shield of cryo holder when IGP1 <15; you may see a slight increase in pressure, but should
settle within a few seconds
9. Open column valves when IGP < 10.
10. Center preset beams
a. Send stage to square of non-interest (square with carbon that you don’t mind trashing)
i.
Under “square_targeting”, select “acquisition” mouse and select square of non-
interest; submit target (click green “play” button)
b. Once the stage stops moving, check that beam is centered at all presets
i. Center grid preset beam
1. Send scope to grid preset and lower flu screen
2. Center beam using left trackball
3.
Open the “Edit” box and click “apply beam shift from scope”
ii. Repeat step i. for square and hole preset
iii. Center and adjust intensity of FA/FC/EN preset beam
1. Send scope to “EN” preset and lower flu screen
2. Center beam using left trackball
3.
Open the “Edit” box and apply beam shift “from scope”
4.
Apply the same beam shift “from scope” for FA and FC preset
11. Get eucentric height and focus
a. Send scope to square preset and lower flu screen (R1 on knobset)
b. Using Right Knobset joint stick, move stage until you see a grid square with unique
fiducial; center that square on the flu screen
c.
“Z_focus” node→ simulate target (+ icon)
d. Recommended- record the eucentric height value;
it is useful to have an idea of what the
eucentric height of your grid is, especially as you navigate around during data collection; (the
eucentric height should stay relatively similar, +/- 50
this means your grid is fairly flat; if your eucentric height varies much more than that, your grid is
probably bent/wrinkled)
e.
“Z_focus” node → MF (manual focus
12. Collect atlas of your grid (this is a series of low mag images that are stitched together to give you
a whole view of your grid; ~20 minutes)
a.
Under the “Grid_targeting” node, click “settings” button (upper left hand side) and input
your grid description
i. Radius = 0.0009m = 0.9mm