SEMC TF20, Руководство пользователя, Страница: 14 / 30

Last updated 10/29/2020
13
i. Should see bright green small beam on the flu screen with diffuse carbon
diffraction ring around it
c. Insert objective aperture and center
d.
Exit “DIFFRACTION” mode
e. If you plan to take a break, send microscope to high mag (FA/FC/EN) to turn on the high
mag lenses and close the column valves
17. You are now ready to collect some images!
18.
In “Square_Targeting” select 1 square of interest
a.
Using “acquisition” mouse, select the center of square of interest
b.
Click “Submit target”
c.
Click “Submit queued targets”
19.
In “Hole_Targeting” select 1 - 2 regions of holes of interest
a.
Using “acquisition” mouse, select holes of interests (on the carbon, every 2x2 holes
away from each other)
b.
Using “focus” mouse, select area of carbon towards the center of the hole areas you
selected in the step above (this is where leginon will acquire the eucentric height of the
square)
c.
Click “Submit target”
d.
Click “Submit queued targets” when you are done with every hole target
20.
In “Exposure_Targeting” node select exposure targets
a.
Using “acquisition” mouse, select the center of the holes of interests, on the ice. Select
up to 4 holes that are closest to the center of the screen (use “toggle center crosshair” tool
to find where center is). This ensures that you do not collect images with a lot of image
shift.
b.
Using “focus” mouse, select area of carbon towards the center of the stage (use “toggle
center crosshair” tool to find where center is). This is where leginon will acquire auto-
focusing for the exposure targets selected in the step above.
c.
Click “Submit target”
d.
Click “Submit queued targets”
i. Note- at this point you have queued up a number of exposure targets for leginon
to acquire.
21.
Under “Hole” node, select “toggle queue timeout” so that column valves will close once exposure
queue is done collecting
22. To collect a new square, repeat steps 20 - 22 until you are done collecting data.
23. Start frame alignment
a. In emgweb, select the processing link (upper left side)
b. Login with LDAP info (upper right side)
c.
In “Direct Detector Tools” → “Select Frame Alignment” → “Full Frame alignment for
Direct Electron Co. Camera”
d. Input the following parameters.