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Last updated 10/29/2020
17
Gatan Elsa cryo holder manual
The Elsa holder is a specialized holder with a large cryogenic dewar that can maintain temperature
for ~8 hours.
Elsa holder handling should be done by EMG staff only; this includes loading the sample,
insertions, and retractions. Users should only be operating Leginon.
●
1X per week, the holder (V4) should be pumped down
●
1X per month, the holder should be baked out
●
Every day the holder is used for cryo, a warm up should be applied at the end of the day
1. Remove Elsa holder from the dry
pumping station
a. V4 and V3 of Elsa will most
likely be closed; if not, close
these valves first (close V4 and
then V3)
b. If there is a second holder
attached to the dry pumping
station, close V4 and then V3
of the second holder
c. Close V1 and then V2
d. Turn dry pumping station off
e.
Once the “MDP status” and
“System Status” turn off, retract
holder from dry pumping station
f. Transfer Elsa holder to the Elsa cryo transfer station
2.
Cool down the Elsa holder in “Cryo Transfer” mode (should be done on the pumping station
desk)
a. Attach the Elsa holder to the Elsa temperature controller using the 698 cord (thick grey
cord). Make sure to connect red dots to one another.
b.
Switch to “Cryo Transfer” mode and start
c. Slowly add liquid nitrogen to the tip and dewar sides of the holder until you are at -
170℃. This takes ~30 minutes.
3. Transfer sample to the Elsa holder
a. Open the shield of the holder.
b. Once the holder is at -170℃ you can unplug it from the temperature controller and move
the Elsa with its cryo transfer station over to the general bench.
c. Remove the clip ring from the holder tip. Place the silver end of the tool onto the clip ring
and while applying a small amount of vertical pressure on the end of the clip ring tool,
slowly turn the tool clockwise 1 - 2 turns until you feel resistance. *Be careful not to turn
too far as it will over-tighten the clip ring to the clip ring tool*. Pull up to remove the clip
ring.