QwikCheck™
Sperm Quality Analyzer Guide Version 1.00 I-Button WHO 5th 06_AUG_2012
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APPENDIX 8: Treating Viscous Samples
Product Insert
INTRODUCTION AND INTENDED USE
The QwikCheck
TM
Liquefaction Kit can be used to promote and accelerate the liquefaction of
viscous semen samples that remain viscous thirty minutes after collection. High viscosity can
impact the accurate measurement of motility and concentration and antibody coating. The
QwikCheck
TM
Liquefaction Kit is used to prepare viscous semen samples for automated or
manual semen analysis and is for in-vitro use only.
KIT CONTENTS
20 single dose, 5 mg vials of lyophilized α-Chymotrypsin.
Product Insert
STABILITY AND STORAGE CONDITIONS
The product has a one year shelf life. Note the expiration date on the box and vials.
Vials can be stored at room temperature.
INSTRUCTIONS FOR USE
1.
Select one vial of α-Chymotrypsin.
2.
Tap the vial to move the contents to the bottom of the vial prior to opening.
3.
Add the entire contents of one vial to a viscous semen sample.
4.
Gently mix the sample to dissolve the powder.
5.
Once the sample has liquefied (5-10 minutes), immediately perform automated testing
or neutralize the enzymatic activity (optional) by adding of Human Serum Albumin
(HSA) (not provided in this kit).
PRECAUTIONS AND WARNINGS
Each vial contains α-Chymotrypsin, a protease. This protease may cause irritation to eyes,
respiratory system or skin. In case of contact with eyes, rinse immediately with plenty of water
and seek medical attention. Observe the following precautions when handling the product:
Wear suitable protective clothing: Mask, gloves and laboratory coat.
Avoid dispersing material over the working area.
REFERENCES:
WHO Laboratory Manual for the Examination of Human Semen, 5th Edition, Cambridge
University Press, 2010
