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QIAcuity Safety and Installation Guide 04/2021
Comments and suggestions
k)
Double positive or double
negative signals
The double positive or double negative signals could have different
root causes. One of the reasons for observing double signal bands
could be suboptimal assay designs, such as cross-hybridization of
probes to unspecific targets or secondary off-target amplification
products. Besides assays-related causes, improper crosstalk
compensation could also be the root cause. An insufficient
compensation or overcompensation of crosstalk from neighboring
channels could also result in extra signal bands. To determine the
main root cause, re-image the plate with 30% less exposure times for
affected channel. If double bands disappear or get much closer to
each other after re-imaging, they are most likely to be caused by
improper crosstalk compensation rather than assay-related issues.
Software
a)
The QIAcuity Software Suite does
not start
Check that the software is installed on the laptop. Check the
operating system. The QIAcuity Software Suite can only be operated
on Windows 7 and Windows 10.
b)
Disc space is critical in the
QIAcuity Software Suite
Delete plates from the plate overview.
c)
User with role Operator forgot
password
Administrator to log-in, new operator can be created. If
administrator forgot the password, contact QIAGEN Technical
Service.
