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QIAcuity Safety and Installation Guide 04/2021

Troubleshooting

General information

This section provides information about what to do if an error occurs while using QIAcuity.

Contacting QIAGEN Technical Services

Whenever you encounter a QIAcuity error, ensure that you have the following information at
hand:



Software version



Sample input material



Detailed description of the error situation



Serial number of the instrument

This information will help you and your QIAGEN Technical Service Specialist to deal most
efficiently with your issue.

Note

: For most cases, to allow proper analysis of an error situation, the support package either

from the instrument and/or the suite is required. Refer to Creating a support package.

Note

: Information about the latest software and protocol versions can be found at

www.qiagen.com/MyQIAcuity

. In some cases, updates may be available for addressing

specific problems.

Содержание QIAcuity 911000

Страница 1: ...Sample to Insight__ April 2021 QIAcuity Safety Information and Installation Guide 911000 911020 911040 911050 QIAGEN GmbH QIAGEN Strasse 1 D 40724 Hilden 1122040...

Страница 2: ...ectrical safety 12 Environment 13 Biological safety 14 Chemicals 15 Maintenance safety 16 Radiation safety 16 Symbols on the QIAcuity 17 General Description 18 QIAcuity Installation 20 Installation en...

Страница 3: ...65 Contacting QIAGEN Technical Services 65 Performing a self check on the QIAcuity instrument 66 Creating a support package with the QIAcuity instrument software 67 Creating a support package with the...

Страница 4: ...4 QIAcuity Safety and Installation Guide 04 2021 Liability Clause 90 Declaration list of China RoHS SJT 11364 2014 91 Document Revision History 94...

Страница 5: ...roducts This information is helpful to other scientists as well as to the researchers at QIAGEN We therefore encourage you to contact us if you have any suggestions about product performance or new ap...

Страница 6: ...ation guide provides information about QIAcuity in the following sections Intended use Safety Information General description QIAcuity Installation QIAcuity Software Suite Installation Connecting the...

Страница 7: ...partitions Calculation of target concentration is determined by referring back to the volume in all analyzable partitions i e partitions which were filled with reactions mix The total number of fille...

Страница 8: ...al requirements Installation Laboratory technicians or equivalent Appropriately trained or experienced personnel familiar with use of computers and automation in general Routine use running protocols...

Страница 9: ...n are available in in Appendix C Informations de s curit and Appendix D Sicherheitshinweise The following types of safety information appear in this manual WARNING The term WARNING is used to inform y...

Страница 10: ...o incorrect maintenance WARNING Risk of personal injury and material damage W2 The QIAcuity is too heavy to be lifted by one person To avoid personal injury or damage to the instrument do not lift the...

Страница 11: ...p objects into the instrument when the plate tray is ejected WARNING Risk of explosion W7 The QIAcuity is intended for use with reagents and substances supplied with QIAGEN kits or other than outlined...

Страница 12: ...ely to expose live parts WARNING Damage to electronics W9 Before powering ON the instrument make sure that the correct supply voltage is used Use of incorrect supply voltage may damage the electronics...

Страница 13: ...trument may be electrically unsafe when It or the line power cord appears to be damaged It has been stored under unfavorable conditions for a prolonged period It has been subjected to severe transport...

Страница 14: ...gical safety Specimens and reagents containing materials from humans should be treated as potentially infectious Use safe laboratory procedures as outlined in publications such as Biosafety in Microbi...

Страница 15: ...e and local health and safety regulations and laws Chemicals WARNING Hazardous chemicals W13 Some chemicals used with this instrument may be hazardous or may become hazardous after completion of the p...

Страница 16: ...ents to come into contact with the electrical parts of the QIAcuity CAUTION Damage to the instrument C8 Do not use bleach solvents or reagents containing acids alkalis or abrasives to clean the QIAcui...

Страница 17: ...estriction of the use of certain hazardous substances in electrical and electronic equipment Type plate on the back of the instrument Waste Electrical and Electronic Equipment WEEE mark for Europe Typ...

Страница 18: ...up to 24 or 96 samples per plate can be analyzed For high sensitivity applications the QIAcuity Nanoplate 26K 24 well is available The number of in parallel processable plates depends on the instrumen...

Страница 19: ...IAcuity Eight always offer 5 plex analysis This table shows the available channels provided in each instrument Table 2 QIAcuity instrument configurations Instrument Available channels QIAcuity One 2 p...

Страница 20: ...idity The site of installation should be free of excessive drafts excessive moisture and excessive dust and should not be subject to large temperature fluctuations Use a level workbench that is large...

Страница 21: ...ation maintain a minimum clearance of 10 cm at the sides and rear of the QIAcuity Slits and openings that ensure the ventilation of the QIAcuity must not be covered C7 WARNING Risk of personal injury...

Страница 22: ...ltage is used Use of incorrect supply voltage may damage the electronics To check the recommended supply voltage refer to the specifications indicated in the type plate of the instrument W9 WARNING El...

Страница 23: ...AC power outlet that has no ground earth connection WARNING Electrical hazard Any interruption of the protective conductor earth ground lead inside or outside the instrument or disconnection of the pr...

Страница 24: ...Acuity Powering ON the QIAcuity Removing the protective film from the QIAcuity hood Carefully peel off the protective film from the QIAcuity hood and the touch screen Removing the shipping fixation sc...

Страница 25: ...ity matches the voltage available at the installation site 3 Connect the power cord to the power inlet on the instrument and connect the cable to the wall power outlet 4 Turn on the power switch at th...

Страница 26: ...ts is likely to expose live parts W5 Powering ON the QIAcuity Check that the QIAcuity operates properly 1 Ensure that the drawer of the QIAcuity is closed 2 Power ON the QIAcuity using the blue front...

Страница 27: ...tionally supplied with a notebook See the following table for the recommended notebook requirements Table 3 Recommended notebook requirements Description Recommended requirement Operating system Micro...

Страница 28: ...standby hibernation settings in the Windows configuration 1a Right click the Windows start menu and select Power Options 1b Click change plan settings next to your current power plan 1c Select Never i...

Страница 29: ...QIAcuity Safety and Installation Guide 04 2021 29 3 Check the I accept the terms in the License Agreement box in the End User License Agreement window and click Next...

Страница 30: ...30 QIAcuity Safety and Installation Guide 04 2021 4 Click Next in Destination Folder window...

Страница 31: ...QIAcuity Safety and Installation Guide 04 2021 31 5 Click Install to start the installation 6 In the prompt window asking you if you allow changes to your device click Yes...

Страница 32: ...32 QIAcuity Safety and Installation Guide 04 2021 Note During installation some command line windows may appear in the foreground Do not close any of them...

Страница 33: ...QIAcuity Safety and Installation Guide 04 2021 33 7 When the installation is completed click Finish 8 In the local host site that says the site is not secure click Details...

Страница 34: ...34 QIAcuity Safety and Installation Guide 04 2021 9 Click Go on to the webpage You are directed to the QIAcuity Software Suite login screen...

Страница 35: ...QIAcuity Software Suite The QIAcuity instrument needs to be connected to the QIAcuity Software Suite to enable the exchange of data This also enables the QIAcuity Software Suite to set up plates anal...

Страница 36: ...nected to a LAN local area network This configuration allows the QIAcuity notebook or a separate computer to work as a server to which multiple clients can be connected The QIAcuity Suite server insta...

Страница 37: ...make sure that the QIAcuity instrument is connected to the LAN Any other configurations are not supported by QIAGEN If you are unsure about your network infrastructure please consult with your local I...

Страница 38: ...Internet if not available proceed to the next step 12 Click Network and Sharing Center 13 On the left pane click Change adapter settings 14 Right click the ethernet network adapter and select Properti...

Страница 39: ...afety and Installation Guide 04 2021 39 16 Select Obtain an IP address automatically If your organization does not provide DNS details select Obtain DNS server address automatically as well Click OK t...

Страница 40: ...e been assigned properly by following the steps below 1 From your Home screen click the Search icon Enter cmd and press the Enter key 2 Wait for the command line window to open Enter ipconfig command...

Страница 41: ...QIAcuity Safety and Installation Guide 04 2021 41 Follow the steps below to configure the QIAcuity instrument 1 On the toolbar tap Configuration 2 Select the Ethernet tab...

Страница 42: ...tion Guide 04 2021 3 Check the DHCP enabled box If you check this box the IP address and MAC address fields are disabled The assigned IP and MAC addresses of device are displayed in the IP address and...

Страница 43: ...etween the QIAcuity instrument and the QIAcuity Software Suite Note Before you start ensure that the QIAcuity instrument and the notebook are connected with an Ethernet cable Follow the steps below to...

Страница 44: ...rnet if not available proceed to the next step 3 Click Network and Sharing Center 4 On the left pane click Change adapter settings 5 Right click the ethernet network adapter and select the Properties...

Страница 45: ...92 168 1 1 Subnet mask Enter 255 255 255 0 Default gateway Enter 192 168 1 254 Preferred DNS server Enter the DNS server address Alternative DNS server Enter the alternative DNS server address Note If...

Страница 46: ...have been assigned properly by following the steps below 9 From your Home screen click the Search icon Enter cmd and press the Enter key 10 Wait for the command line window to open Enter ipconfig Addr...

Страница 47: ...ow to configure the QIAcuity instrument 1 On the toolbar tap Configuration 2 Select the Ethernet tab 3 Ensure that the DHCP enabled box is not checked Enter the following information IP address Enter...

Страница 48: ...ent and the device in which the QIAcuity Software Suite is running must be connected to the same network Note A plate run can only be performed if the QIAcuity Software Suite is connected to the instr...

Страница 49: ...QIAcuity Safety and Installation Guide 04 2021 49 2 On the Home screen tap Configuration 3 Tap the Software Suite tab...

Страница 50: ...e IP address of the Suite Server the instrument must be connected to the Suite Server From your Home screen click the Windows icon and navigate to Command Prompt or enter cmd in the Search field A com...

Страница 51: ...ity Safety and Installation Guide 04 2021 51 Note An error message displays if the URL you entered is invalid Tap OK to close the error message then re enter the correct URL in the Software Suite URL...

Страница 52: ...figure the proxy settings in your network configuration clear the Do not use a proxy box and enter the following information Proxy Server Enter the proxy server address Proxy Port Enter the port addre...

Страница 53: ...enance Periodic maintenance Optionally these procedures may be performed to check and ensure the reliability of operation of the QIAcuity Select the cleaning agent according to the objective of the cl...

Страница 54: ...lar to those described below General cleaning of the QIAcuity Mild Detergents e g Mikrozid AF sensitive 25 ethanol Disinfection Ethanol based disinfectants can be used for disinfection of surfaces e g...

Страница 55: ...d General instructions Do not use spray bottles to spray cleaning or disinfectant liquids onto surfaces of the QIAcuity If solvents or saline acidic or alkaline solutions are spilt on the QIAcuity wip...

Страница 56: ...k of fire W24 Do not allow cleaning fluid or decontamination agents to come into contact with the electrical parts of the QIAcuity Take special care while cleaning the extended drawer that no liquid i...

Страница 57: ...Service specialist WARNING Risk of explosion W23 When cleaning the QIAcuity with alcohol based disinfectant allow flammable vapors to disperse WARNING Risk of fire or explosion W23 When using ethanol...

Страница 58: ...gular maintenance procedure of QIAcuity Clean the instrument on a regular basis especially if fluids have been spilled on the instrument See Cleaning agents for the recommended cleaning agents you can...

Страница 59: ...and Installation Guide 04 2021 59 1 Turn off instrument and remove power cord 2 Reach under the front of the instrument and push both buttons simultaneously 3 Remove the filter from the swing out fil...

Страница 60: ...artment to the top to close Calibration of thermal cycler The thermal cycler is designed to operate with the same specifications over the lifetime of the instrument To ensure and verify the quality of...

Страница 61: ...nance procedure for QIAcuity Instrument Software The QIAcuity stores various information about the runs and plates used in the instrument Images created during the runs are deleted automatically after...

Страница 62: ...Only administrators can delete data from the instrument 1 Tap the Tools icon 2 Tap Data Management 3 To clear the data tap Force clear images Click OK in the confirmation dialog box to delete the dat...

Страница 63: ...e space of your disk click Disk monitor in the main toolbar This shows an overview about the state of the disk disk name and disk path It also shows the remaining free space and total space of your di...

Страница 64: ...warning level there is only disk space for a few runs left Yellow dot in disk monitor icon Critical No disk space left to store more run data Red dot in disk monitor icon Unavailable The disk is not...

Страница 65: ...etailed description of the error situation Serial number of the instrument This information will help you and your QIAGEN Technical Service Specialist to deal most efficiently with your issue Note For...

Страница 66: ...movement All modules return to their initial positions If a plate is detected in the gripper the plate is returned to the drawer To start a self check follow these steps 1 Tap Tools 2 Tap Self check...

Страница 67: ...d it to the Software Suite follow the steps below The QIAcuity Software Suite will combine the support package from the instrument and the Software Suite 1 On the toolbar tap Tools 2 Tap Data Manageme...

Страница 68: ...om timeframe tap From and select a start date from the calendar Then tap To and select an end date from the calendar 6 Tap Apply to save the changes Figure 5 Set Support Package Timeframe dialog box 7...

Страница 69: ...e a support package and save it on a USB drive follow the steps below 1 On the toolbar tap Tools 2 Tap Data Management 3 Connect a USB drive to the instrument Wait for the device to detect the USB dri...

Страница 70: ...eframe is the last two weeks 6 Tap the applicable option for your preferred timeframe Tap Today to create a support package for the current day Tap Last week or Last 2 weeks to select either the last...

Страница 71: ...llation Guide 04 2021 71 8 Tap Create download A progress bar is shown To cancel the download tap the progress bar Once the download is complete a notification is displayed and the USB drive can be re...

Страница 72: ...support package with the QIAcuity Software Suite 1 Select a plate in the plate overview 2 Click the left side of the screen on Support Package A new window pops up where you can specify what kind of s...

Страница 73: ...s and a combined image view of all wells The extended package includes the log files and the raw images of each single well By clicking on the calendar symbol you can define the time frame from which...

Страница 74: ...ing an operation please ensure correct ventilation of the instrument and correct environmental conditions according the installation chapter requirements Please ensure that the air filter is not clogg...

Страница 75: ...and level surface as described in Installing the QIAcuity b Scratches appear on the instrument Always use the cleaning products as described in Maintenance Procedures Do not use bleach or ethanol as...

Страница 76: ...PC This will lead to complete data loss on the USB stick and it can no longer be used with the QIAcuity f Starting of instrument takes long Depending on precondition start could take up to 5 minutes...

Страница 77: ...case re image the plate with 30 higher exposure time or gain settings f Sample results of replicates differ a lot Check the images for blacked out areas that can occur e g due to bad filling or areas...

Страница 78: ...also result in extra signal bands To determine the main root cause re image the plate with 30 less exposure times for affected channel If double bands disappear or get much closer to each other after...

Страница 79: ...IAcuity instrument or the notebook where the QIAcuity Software Suite is installed f Startup of instruments displays an error The required plate recovery task cannot be performed because there are no p...

Страница 80: ...ror occurs Details about errors that occurred on the instrument are shown in the System Status section After viewing the information administrators can clear errors and restart the instrument to initi...

Страница 81: ...ity Eight the More details button is located in the Upper Tray and Lower Tray panes In the QIAcuity Four and QIAcuity One the More details button is located in the Tray pane 6 Restart the instrument T...

Страница 82: ...he objective of the cleaning procedure the sample material used and the downstream assay WARNING Risk of fire or explosion W1 When using ethanol or ethanol based liquids on the QIAcuity handle such li...

Страница 83: ...ains supply voltage fluctuations are not to exceed 10 of nominal supply voltages Maximum power consumption QIAcuity One 2plex 1000VA QIAcuity One 5plex 1000VA QIAcuity Four 1000VA QIAcuity Eight 1500V...

Страница 84: ...84 QIAcuity Safety and Installation Guide 04 2021 Pollution level 2 Environmental class 3K21 IEC 60721 3 3...

Страница 85: ...s package Relative humidity 5 to 85 non condensing Environmental class 2K11 2M4 IEC 60721 3 2 Ambient pressure 700hPa to 1060hPa Storage conditions Air temperature 5 C to 40 C 41 F to 104 F in manufac...

Страница 86: ...idth 38 cm 15 0 in Height 45 cm 17 7 in Depth 65 cm 25 6 in Mass QIAcuity One 36 0 kg 79 4 lb QIAcuity Four 43 0 kg 94 8 lb QIAcuity Eight 55 0 kg 121 3 lb Accessories 3 0 kg 6 6 lb Thermal specificat...

Страница 87: ...les per plate Maximum plate capacity depends on the configuration One Four Eight Touchscreen Four Eight 10 1 LCD Touch active area 218 0 x 136 6 mm resolution 1280 800 HD Touchscreen One 7 0 LCD Touch...

Страница 88: ...l Scan Pattern Area Image 1280 x 800 pixel array Motion Tolerance Up to 89 cm s 35 in s Print Contrast Ratio 15 minimum Decode Capability Reads standard 1D 2D Postal and stacked codes Resolution 1D Li...

Страница 89: ...isposal of waste electrical and electronic equipment by users The crossed out wheeled bin symbol see below indicates that this product must not be disposed of with other waste it must be taken to an a...

Страница 90: ...birth defects and or other reproductive harm For more information go to www P65Warnings ca gov Liability Clause QIAGEN shall be released from all obligations under its warranty in the event repairs o...

Страница 91: ...aration list of China RoHS SJT 11364 2014 The environmental friendly use period of the QIAcuity instruments is 25 years The marking for the Restricted Use of Hazardous Substances in Electronic and Ele...

Страница 92: ...ead Pb Mercury Hg Cadmium Cd Hexavalent Chromium Cr6 Polybrominated biphenyls PBB Polybrominated diphenyl ethers PBDE Plastics Enclosure Plastics parts O O O O O O Mechanical units Chassis Moving part...

Страница 93: ...O O O O O Filter Lenses O O O O O O Heating Unit Thermocycler O O O O O O Note This table is prepared in accordance with the provisions of SJ T 11364 O Indicates that this toxic or hazardous substanc...

Страница 94: ...94 QIAcuity Safety and Installation Guide 04 2021 Document Revision History Date Changes April 2021 Initial release...

Страница 95: ...hird parties 2 Other than expressly stated licenses QIAGEN makes no warranty that this kit and or its use s do not infringe the rights of third parties 3 This kit and its components are licensed for o...

Страница 96: ...96 QIAcuity Safety and Installation Guide 04 2021 Ordering www qiagen com shop Technical Support support qiagen com Website www qiagen com...

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