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Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516

www.promega.com

TM320 · Revised 4/15

All technical literature is available at: www.promega.com/protocols/

Visit the web site to verify that you are using the most current version of this Technical Manual.

E-mail Promega Technical Services if you have questions on use of this system: [email protected]

Maxwell

16 MDx

Instrument Operating Manual

1. Introduction

............................................................................................................................................3

1.A. Maxwell

16 MDx Purification Procedure .........................................................................................3

1.B. Maxwell

16 MDx Instrument Specifications ....................................................................................4

2. Hardware Overview

.............................................................................................................................. 10

3. Hardware Mode

.................................................................................................................................... 13

4. Unpacking and Setting Up the Maxwell

16 MDx Instrument

.............................................................. 14

4.A.  Unpacking ..................................................................................................................................... 14
4.B.  Removing the Magnetic Rod Assembly/Plunger Bar Platform Shipping Anchors .............................. 14
4.C.  UV Bulb Installation ...................................................................................................................... 15
4.D.  Maxwell

Sample Track Software ................................................................................................... 16

4.E. Operational Setup .......................................................................................................................... 16

5. Operating the Maxwell

16 MDx Instrument

....................................................................................... 23

6. Run Reports

.......................................................................................................................................... 31

6.A.  Data File Transfer at the End of a Run ............................................................................................ 31
6.B.  Data File Transfer at a Later Time .................................................................................................. 32
6.C.  Data File Management ................................................................................................................... 32

7. Changing Configuration Parameters

..................................................................................................... 32

7.A. Changing the Hardware ................................................................................................................. 33
7.B. Changing the Instrument Firmware Configuration .......................................................................... 36

Содержание Maxwell 16 MDx

Страница 1: ...Revised 4 15 TM320 O P E R A T I N G M A N U A L Maxwell 16 MDx Instrument Instructions for Use of Product AS3000 2800 Woods Hollow Rd Madison WI USA...

Страница 2: ...E Precautions 7 1 F Environmental Requirements operating shipping and storage conditions 9 2 Hardware Overview 10 3 Hardware Mode 13 4 Unpacking and Setting Up the Maxwell 16 MDx Instrument 14 4 A Unp...

Страница 3: ...n Using the UV Lamp 38 8 E Replacing the UV Bulb 39 8 F Periodic Maintenance 39 9 Troubleshooting 40 10 Appendix 45 10 A User Defined Methods 45 10 B Homogenization Method 52 10 C Updating Firmware 53...

Страница 4: ...es bound to the particles away from other cellular components Elution of the product The instrument contains a touch screen for navigating programming and running the instrument It has a UV lamp to ai...

Страница 5: ...mination of instrument Reporting functionality Maxwell Sample Track Software and bar code reader included Preprogrammed methods for DNA RNA and protein purification Ability to create user defined prot...

Страница 6: ...ck Start Guide 1 Bar Code Reader provided in a separate box 1 CD containing Sample Track Software and manual 1 RS 232 Cable for communication with a computer or printer 1 RS 232 USB Adaptor for data e...

Страница 7: ...090TA 11470TA 11546TA Figure 1 Maxwell 16 MDx Instrument accessories Panel A Components shown include Bar Code Reader Cable Bar Code Reader Power Cord UV Bulb SD Card Reader and Cable SD Card RS 232 U...

Страница 8: ...hazard to the instrument or surrounding area Warning Pinch point hazard Warning Hot surface Burn hazard Warning Lifting hazard Warning Biohazard Warning UV light hazard Do not look directly at the UV...

Страница 9: ...o do so may result in damage to the instrument After each run verify that the plungers have been completely removed from the magnet rods before pressing Run Stop to extend the platform Use only Promeg...

Страница 10: ...Install the instrument on a clean level surface To avoid shortening the expected lifespan of the instrument install in a location that meets the following criteria Locate on a sturdy level surface Avo...

Страница 11: ...ont view of the Maxwell 16 MDx Instrument 8085TA Figure 3 Touch Screen and Keypad The touch screen and the keypad for use in navigating through the various screen displays for the Maxwell 16 MDx Instr...

Страница 12: ...the rear of the Maxwell 16 MDx Instrument The rear of the instrument showing the On O switch and where to connect the power cable A 3 amp time lag fuse is located next to the power switch 8082TA On o...

Страница 13: ...ion 10 C The SD card slot cannot be used to download data from the Maxwell 16 MDx Instrument We recommend that you keep the SD Card in the instrument to avoid misplacing it Figure 6 shows the hardware...

Страница 14: ...he various Maxwell Purification Kits for information on the hardware mode setting required The existing hardware mode SEV or LEV setting is displayed in the upper right hand corner of the Home screen...

Страница 15: ...he packaging material in case the instrument needs to be returned for service or repair later 4 B Removing the Magnetic Rod Assembly Plunger Bar Platform Shipping Anchors 1 Ensure that the instrument...

Страница 16: ...t see Figure 10 Twist the bulb a quarter turn until it is firmly in place The UV bulb has an average life of 3 000 hours The Maxwell 16 MDx Instrument will warn you when the accumulated UV bulb usage...

Страница 17: ...e Tripp Lite instruction manual Connect the bar code reader to the USB port on the side of the instrument Users who wish to print run data directly can connect a serial printer to the RS 232 port of t...

Страница 18: ...itial setup This Wizard will help you to set up the instrument configuration to best meet your needs During setup you can set the following parameters Language Touch Screen settings Date Time UV Lamp...

Страница 19: ...een Reset Successful message indicates that the calibration was successful and the Wizard can continue Figure 12 Calibrating the Touch Screen To calibrate the screen touch the symbols in the order the...

Страница 20: ...Selecting each of the screen options opens a keypad to add numerical values 8207TA Figure 14 Date Time Setup screen 4 UV Lamp You can program the Maxwell 16 MDx Instrument to turn on the UV lamp auto...

Страница 21: ...e Options screen Important The options chosen must be entered during the run setup Options not chosen will be displayed on the Barcode Input screen during run setup Section 5 B but you will not be abl...

Страница 22: ...the user to enter a PIN to perform a purification run The PIN is four digits The list of users and associated PINs can be modified as described in Section 7 B Keep a separate list of all users and ass...

Страница 23: ...is treatment Important Do not open the door if the UV lamp is on The UV lamp will not run if the door is open The Home screen contains the menu options that allow access to all of the functions availa...

Страница 24: ...ocol 8231TA 2 Choose a protocol according to the Maxwell reagent kit technical manual Select User to access and run custom protocols Instructions for setting up customized protocols are presented in S...

Страница 25: ...5 B for more information Setting Up a Run in the LEV Mode 1 The Home screen must indicate LEV Select Run Select the User and add the matching PIN if appropriate Pressing the Run Stop button opens the...

Страница 26: ...o the front position of the LEV rack Once all of the samples elution tubes and plungers have been loaded press the Run Stop button The instrument platform will move back into the instrument Close the...

Страница 27: ...al position To start at a di erent position number select the Position option to open a keypad for choosing the required number 2 Scan the bar code label on the sample tube The bar code data is displa...

Страница 28: ...ion All of the data can be removed by selecting the Clear All option 7 Select Next Sample to add the information for the next sample For empty sample positions select Next Sample repeatedly until the...

Страница 29: ...the data required The data will appear on the screen next to Eluate ID Place the tube in the appropriate elution tube slot at the front of the cartridge rack next to the cartridge Review the data All...

Страница 30: ...trument goes through a run without plungers the magnetic rod assembly must be removed and cleaned see Section 8 B The cartridges containing the samples must be discarded During the run the Processing...

Страница 31: ...thod has ended The export of run data occurs before the samples are removed from the instrument see Section 6 8222TA After the run is complete open the instrument door and check that all of the plunge...

Страница 32: ...xport If No is selected the run data are saved for subsequent retrieval and not exported If Yes is selected the File Output screen opens 8223TA The Transfer to PC option allows the computer containing...

Страница 33: ...ile from the instrument To remove files use the Data Transfer option on the Home screen In the File Output screen select File Maintenance and enter the Administrator PIN In the Select File screen the...

Страница 34: ...ll 16 Configuration screen 2 Follow the on screen instructions First open the door 3 The Magnetic Assembly will move down to allow you to remove the Magnetic Assembly Rod Do not push down on the magne...

Страница 35: ...l hardware installed If the screen does not match the physical hardware installed turn the instrument o install the appropriate hardware and restart the machine The hardware mode indicated on screen s...

Страница 36: ...tighten 9 Close the door the screen will indicate that the hardware has been changed The instrument indicates a hardware change once the door is closed whether or not you actually completed the physi...

Страница 37: ...from the Maxwell 16 Configuration screen Important You may need to scroll down the screen to see all of the options 8284TA 1 Changing the Language Select the Select Language option in the Configurati...

Страница 38: ...samples or reagents have been spilled it is important to clean the instru ment immediately to avoid damage or contamination of samples Most parts of the Maxwell 16 MDx Instrument have an anodized coat...

Страница 39: ...is removed In case of spills in the instrument where there is a potential biohazard wipe up the spill with towels and wash the spill area with a detergent solution such as Steris LpH se following the...

Страница 40: ...in the UV lamp socket which is just inside the door on the ceiling Twist the bulb a quarter turn until it can slide out Place the new bulb into the instrument see Figure 10 UV lamp socket as described...

Страница 41: ...located next to the power switch If the fuse is blown iden tify and correct the cause Never replace this fuse with a fuse rated for higher than 3A Contact Promega or your local Promega representative...

Страница 42: ...temperature before continuing the run This means that there may be slight variations in run time between runs UV Lamp errors After 2950 hours of accumulated lamp time the following error message appea...

Страница 43: ...t reached the set point in a few minutes there may be a problem with the heater Contact Promega or your local Promega represen tative for service A heater problem may a ect yield Installed hardware do...

Страница 44: ...forward freely Turn the in strument o wait a few seconds and turn the instrument back on If the calibration error persists please contact Promega or your local Promega representative for service Calib...

Страница 45: ...anually move the plungers up and down in the wells to wash the particles o Switch the instrument on Note the UV light will not come on during the self diagnostic checks that the instrument performs Ho...

Страница 46: ...ysis times could result in the formation of precipitates which may interfere with purification and downstream applications Changing Binding Time This is the amount of time that is allowed for binding...

Страница 47: ...Maxwell Instrument Settings for Preprogrammed Methods The values for the Maxwell 16 MDx Instrument preprogrammed methods are given in Table 1 for LEV and Table 2 for SEV These can be used as a guide...

Страница 48: ...promega com TM320 Revised 4 15 Setting a User Defined Method 1 To set up the custom method select Setup in the Home screen In the Maxwell 16 Configuration screen select User Protocols 8265TA 2 In the...

Страница 49: ...nued 3 In the User Protocol screen select Edit The Choose A Method screen will appear choose RNA DNA or Protein SEV or DNA RNA or Total NA LEV 8235TA 4 In the User Protocol screen select Edit Protocol...

Страница 50: ...method parameters are defined the new method can be named and a PIN added PIN protec tion can prevent inadvertent changes After changing the parameters press the Run Stop button to open the User Proto...

Страница 51: ...and press the Run Stop button 8234TA 2 In the User Protocol screen select View to see the parameters for an existing method Select Edit and enter the correct PIN if the method is PIN protected 8235TA...

Страница 52: ...next User Protocol screen select Administration to open the User Protocol Options screen 8237TA 5 To change the protocol name select Edit Add Protocol Name use the keyboard to enter the new name To ch...

Страница 53: ...s in the lysis bu er If the sample floats use 200 l of lysis bu er If the sample sinks use 300 l of lysis bu er Sample Heating The sample can be heated during homogenization to enhance the release of...

Страница 54: ...on your computer Place the SD card containing the firmware into the SD card port make sure the instrument door is closed and turn the instrument o and on The instrument will install the firmware and t...

Страница 55: ...which describes how to do this The Utilities Menu screen also allows the following options Run Count shows the number of times the instrument has been run UV Bulb Time shows the number of hours the b...

Страница 56: ...esult in a decontamination charge Use the original packaging to ensure that no damage will occur to the instrument during shipping Any damage will incur additional charges Note If the original packagi...

Страница 57: ...f the open box Slide the small inside shipping box containing the instrument into the large outside shipping box Repack the Maxwell 16 MDx Instrument accessories into the original box and place on top...

Страница 58: ...n addition to the standard limited warranty that comes with the Maxwell 16 MDx Instrument a premier warranty and various service agreement options are available for purchase If you purchased a premier...

Страница 59: ...n We will repair your instrument and return it to you performing to original factory specifications Additionally this option includes one preventive maintenance visit during the term of the agreement...

Страница 60: ...ctionality and demonstrates that everything ordered with your instrument is supplied and installed in your laboratory Upon delivery to the lab the instrument and its components will be visually inspec...

Страница 61: ...40 Maxwell 16 LEV Blood DNA Kit 48 preps AS1290 Maxwell 16 Buccal Swab LEV DNA Purification Kit 48 preps AS1295 Maxwell 16 LEV Plant DNA Kit 48 preps AS1420 RNA Purification Kits Product Size Cat Maxw...

Страница 62: ...from the instrument surfaces Repeat the procedure as many times as required to e ectively disinfect and decontaminate the instrument Failure to confirm disinfection and decontamination will result in...

Страница 63: ...ed trademarks of Steris Inc Tripp Lite is a registered trademark of Trippe Manufacturing Company Products may be covered by pending or issued patents or may have certain limitations Please visit our W...

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