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Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516
TM320 · Revised 4/15
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10.A. User-Defined Methods (continued)
Increasing Number of Cycles:
This is the number of times the entire process will be repeated.
•
Potential Benefits:
Increasing this can result in greater recovery of target molecules.
•
Potential Disadvantages:
Increasing this can result in the transfer of detergents, contaminants and other
components that may inhibit downstream applications.
Default Maxwell
®
Instrument Settings for Preprogrammed Methods
The values for the Maxwell
®
16 MDx Instrument preprogrammed methods are given in Table 1 for LEV and Table
2 for SEV. These can be used as a guide for selecting values for custom methods
Table 1. Default LEV Method Parameters (RNA)
.
Step
RNA
Lyse
3.00 minutes
Bind
2.00 minutes
Dry
3.00 minutes
Elute
5.00 minutes
Cycles
1
Table 2. Default SEV Method Parameters.
Step
DNA from
Blood or Cells
DNA from
Tissue
DNA from
Buffy Coat
RNA from
Tissue or Cells
Protein (all
Samples)
Lyse
1.67 minutes
10.00 minutes
1.67 minutes
0.25 minutes
1.50 minutes
Bind
1.50 minutes
1.50 minutes
1.50 minutes
3.00 minutes
1.50 minutes
Dry
3.00 minutes
3.00 minutes
7.00 minutes
2.00 minutes
0 minutes
Cycles
2
2
2
2
2
