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Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516
TM320 · Revised 4/15
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5.B. Setting Up Samples and Collecting Input Data (continued)
Capturing Run Data Manually
You can collect run data manually. After you have completed the steps in Section 5.A, open the instrument door,
and the platform will extend.
1.
In the
Barcode Input
screen, select the
S
ample ID
option to add information.
Select Manual
. Use the
keyboard to type in the data required. The data will appear on the screen next to
Sample ID
.
2.
Select the
Cart. ID
option, and the cartridge to which the sample will be added, and select
Manual
. Use
the keyboard to type in the data required. The data will appear next to
Cart. ID
.
3.
For SEV methods, add the sample to the associated cartridge and place the cartridge, in the instrument posi-
tion corresponding to the
Position
number on the screen. Select the
Eluate ID
option, and select
Manual
.
Use the keyboard to type in the data required. The data will appear on the screen next to
Eluate ID
. Place
the tube in the appropriate elution tube slot at the front of the platform next to the cartridge. Review the
data. All data should match. If they do not match, you can reselect the option to change the information.
4.
For LEV methods, select the
Cart. ID
option and the cartridge to which the sample will be added. Select
Manual
. Use the keyboard to type in the data required. The data will appear on the screen next to
Cart. ID
; place the cartridge into the cartridge rack. Add the sample to the cartridge. Select the
Eluate
ID
option, and select
Manual
. Use the keyboard to type in the data required. The data will appear on the screen
next to
Eluate ID
. Place the tube in the appropriate elution tube slot at the front of the cartridge rack next
to the cartridge. Review the data. All data should match. If they do not match, you can reselect the option to
change the information.
5.
Select the
Kit Info
option, and select
Manual
. Add the kit information using the keyboard. The data appear
on the screen next to
Kit Info
. Check that the kit expiration date has not passed.
6.
On-screen data can be removed from each option by highlighting the option and selecting the
Clear Selection
option. All of the data can be removed by selecting the
Clear All
option.
7.
Select
Next Sample
to move to the next position. The appropriate
Position
number will be displayed, and
the kit box information will be carried over to the next position screen. You will not need to add the kit box
data each time. If more than one kit lot is used, the new data can be added once the cartridge and elution
tube from the new kit have been added to the rack.
8.
Repeat Steps 2 to 5 for the next sample and associated cartridge and elution tube and place in the required
position. Select
Next Sample
. Repeat until all samples are loaded.
9.
If there are less than 16 samples, select
Final Sample
when all of the samples are loaded. This will open
the
Verify Scanned Data
screen. This screen will allow you to review the data. If any incorrect data are
identified for a sample, you can use the navigation buttons to return to the
Barcode Input
screen where
Position 01 will be shown. Use
Next Sample
to navigate to the required sample, and add all of the data for
the sample.
10. For SEV runs, once all of the data have been verified, press the Run/Stop button.
11. For LEV runs, once all of the data have been verified, place the cartridge rack into the instrument, and press
the Run/Stop button.
