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Preventing contamination
To prevent contamination of the chamber, select a suitable installation site.
Avoid locations with high temperatures or humidity.
Avoid locations with high temperatures or humidity, because of a greater presence of microorganisms in
the air.
Avoid locations with passers-by or drafts.
Avoid locations near doors, air conditioners, fans, etc., where passers-by or drafts can facilitate the entry of
microorganisms into the chamber.
If possible, use a cleanroom.
To achieve a better culture, it is recommended that a cleanroom be used if one is available.
Use clean containers.
The greatest cause of contamination is dirty containers for cultures. Be careful not to get containers or
trays dirty when taking them in and out.
Keep the chamber clean.
Wipe off any fingerprints. If water spills from the humidifying pan, or if the doors are left open for a long
time, condensation may form on the inside of the doors. If that occurs, wipe off the condensation with a dry
sterile gauze. In particular, clean and disinfect the chamber if the culture medium is spilled. For details,
refer to “ROUTINE MAINTENANCE” on page 87.
Use sterile distilled water in the humidifying pan.
Always use sterile distilled water in the humidifying pan. Do not use ultrapure water, because it may cause
red rust-like particles in the humidifying pan. Clean the humidifying pan once a month. In some cases, an
antibacterial ingredient may precipitate in the water for humidifying. This is not a malfunction.
Keep the incubator out of direct airflows from air conditioners or fans.
Cool airflow from an air conditioner may cause condensation and lead to possible contamination.
