PerkinElmer Raman Micro 200 Series, Руководство по началу работы

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96 . RamanMicro 200 Series Getting Started Guide
SOP-11: Wavelength Calibration Verification of the
RamanMicro 200
NOTE: Wavelength Calibration Verification can be carried out using any Beam path. In this
example, the Microscope 50x Beam path is used.
1. Ensure the instrument is switched on by following SOP-1.
2. Select the 50x objective lens on the microscope.
3. Open the Scan and Instrument Setup dialog.
4. On the Hardware tab select Microscope 50x from the Beam path drop-down list.
5. Click Spectral Preview.
6. Focus on a sample of polystyrene (L1181228).
7. When the polystyrene sample is optimally focused, close the Scan and Instrument Setup
dialog by clicking Exit.
8. Ensure that Dark Field (DF) is selected as the switch position on the Olympus illuminator
module.
9. Cover the microscope with dark cloth to minimize stray light from interfering with the
calibration and ensure that caps are placed on any external fiber optic probes or
accessories.
10. Select Validate and then Instrument from the Instrument menu in Spectrum.
The Validation / Calibration dialog is displayed.
11. Select the Verification tab.