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82 .

RamanMicro 200 Series Getting Started Guide

If this glass fluorescence is a problem and it is not possible to use quartz containers, then it

is possible to acquire a spectrum of the container, and spectrally subtract this from any

spectra containing this fluorescent signature.

Sampling Slurries/Highly absorbing species

Please refer to

Sampling Liquids

. For sample immersion care taken in focusing the probe

within the sleeve can result in a dramatic improvement in spectral quality.

Sampling Gases

Although Raman scattering from gas samples is very weak, the FOP can be used to measure

gas phase under long acquisition times.

WARNING

The standard FOP is not designed for immersion use. The FOP is

sealed against minor splashes. However, immersion in solvents will

cause serious damage. Ensure an immersion sleeve is in place before

immersing your FOP; see Figure 29. Please contact PerkinElmer if you

have any questions in relation to sampling using the FOP.

Содержание Raman Micro 200 Series

Страница 1: ...RamanMicro 200 Series Getting Started Guide MOLECULAR SPECTROSCOPY...

Страница 2: ...t not limited to the implied warranties of merchantability and fitness for a particular purpose PerkinElmer shall not be liable for errors contained herein for incidental consequential damages in conn...

Страница 3: ...00 33 The Czerny Turner Spectrograph 34 Preparing Your Facility 35 Specifications 36 PC Requirements 37 Hardware Requirements 37 Software Requirements 37 Windows Administrator Level 38 Finding a Suita...

Страница 4: ...using a Fiber Optic Probe 92 Calibration and Performance Verification SOPs 94 SOP 10 Calibration Performance Verification 94 SOP 11 Wavelength Calibration Verification of the RamanMicro 200 96 SOP 12...

Страница 5: ...Introduction...

Страница 6: ...Instrumentation This chapter contains a brief overview of the history and theory of Raman spectroscopy Preparing your Facility This chapter shows you how to position your RamanMicro 200 and what util...

Страница 7: ...have to press two keys at the same time for example ALT F All eight digit numbers are PerkinElmer part numbers unless stated otherwise The term RamanMicro 200 Series means the RamanMicro 200 and the R...

Страница 8: ...o en otros En recuadros ste se proporciona informaci n sobre este tipo de circunstancias Caution Attention Nous utilisons le terme CAUTION ATTENTION pour signaler les situations susceptibles de provoq...

Страница 9: ...os recuadros como ste se proporciona informaci n sobre este tipo de circunstancias Warning Danger Nous utilisons la formule WARNING DANGER pour avertir des situations pouvant occasionner des dommages...

Страница 10: ...10 RamanMicro 200 Series Getting Started Guide...

Страница 11: ...Safety Practices...

Страница 12: ...ed to be correct and complete PerkinElmer shall not be liable for errors contained herein nor damages concerned with the use of this document PerkinElmer does not accept liability for the interpretati...

Страница 13: ...of EC directive 2006 95 EC Only use the RamanMicro 200 microscope indoors and under the following conditions Temperature 15 C to 30 C Relative Humidity 75 maximum non condensing Avoid any adjustment...

Страница 14: ...titude up to 2000 m Ambient temperatures of 5 C to 40 C A maximum ambient relative humidity of 80 for temperatures up to 31 C decreasing linearly to 50 relative humidity at 40 C Mains fluctuations not...

Страница 15: ...Safety Ensure that there is at least 15 cm 6 between any cooling vents and adjacent equipment or walls Ensure that the power supply unit PSU vents or fan do not become obstructed Figure 1 Figure 1 Pow...

Страница 16: ...urrent please review the rating plate on the PSU Table 1 PSU input voltages and mains fluctuations information Input Voltage Mains Fluctuations 100 240 VAC 10 of the nominal voltage WARNING Under no c...

Страница 17: ...r the country listed as the shipping destination WARNING If the power cord is not appropriate for your country it must be removed from use immediately and replaced If the power cord has any physical d...

Страница 18: ...ies complies with the EMC standard EN61326 EMC standard for electrical equipment for measurement control and laboratory use and EN55011 ISM Class A rf emissions FCC rules and regulations This product...

Страница 19: ...operation and contact PerkinElmer for advice before using the instrument again The RamanMicro 200 emits a focused beam of laser radiation at 785 nm 250 mW max and is classified as a Class 3B laser sy...

Страница 20: ...n You must only operate Class 3B lasers in a designated area 2 You must fit each Class 3B device with a captive key control switch such that the key cannot be removed from the lock except in the OFF p...

Страница 21: ...t the laser light scattered from a reflective surface or sample Avoid direct exposure to the laser light Place warning signs near the laser operating areas Control laser areas and limit access to indi...

Страница 22: ...ot disconnect the Raman module Do not fit binocular eyepieces to the microscope module These adjustments may deflect laser light from the safe light path through the system WARNING Do not leave blank...

Страница 23: ...ure The LED emission indicator is visible through laser safety goggles The output energy should be enclosed whenever possible using a beam attenuator or cover to avoid unnecessary exposure The laser s...

Страница 24: ...in use when in this position the spacer acts as a lens cap Never look directly into the laser beam or at the laser light scattered from a reflective surface or sample Permanent eye injury may result...

Страница 25: ...uation for a focused beam as given here Figure B5 in ANSI Z136 1 1933 is used to calculate the nominal ocular hazard distance NOHD for the fiber optic probe The equation is 2 1 4 MPE b f NOHD o o Wher...

Страница 26: ...vailable contact PerkinElmer for details In particular the use of an ultra long working distance lens adaptor part number L1320071 provides a working distance of 25 mm Where different focal lengths ar...

Страница 27: ...VELENGHS 785nm THIS LASER PRODUCT CONFORMS TO IEC 60825 1 1993 21 CFR 1040 10 1040 11 LASER RADIATION AVOID EXPOSURE TO BEAM CLASS 3B LASER PRODUCT MAXIMUM OUTPUT 250mW PULSE DURATION CONTINUOUS WAVE...

Страница 28: ...tarted Guide Labels on the Exterior of the Microscope Figure 6 Labels on the microscope LASER APERTURE CAUTION CLASS 3B LASER RADIATION WHEN OPEN AVOID EXPOSURE TO BEAM CAUTION CLASS 3B LASER RADIATIO...

Страница 29: ...Raman History Theory and Instrumentation...

Страница 30: ...observed the scattering from water and alcohol visually In addition to the blue incident light a faint green light also scattered later named Stokes scattering By the 1930s much of the theory underly...

Страница 31: ...Stokes scattering or shorter wavelengths higher frequencies Anti Stokes scattering With Stokes scattering some of the incident photons give up a portion of energy to the molecules and are detected at...

Страница 32: ...tational energy of the molecule The frequency shifts from the frequency of the incident beam are displayed on the x axis of a Raman spectrum generally in relative wavenumbers otherwise known as Raman...

Страница 33: ...ter module by two fiber optic cables The spectrometer module contains the spectrograph and CCD detector The laser supplies light through an objective lens onto the sample The scattered light from the...

Страница 34: ...ting which disperses that light in one dimension The light is then focused onto a photo sensitive CCD detector typically 1024 pixels wide which detects the Raman spectrum Figure 12 Schematic of the Cz...

Страница 35: ...Preparing Your Facility...

Страница 36: ...9 F 30 C 86 F Recommended Temperature 20 C 68 F 25 C 77 F Power Supply 100 240 V 10 50 60 Hz Primary Fuse See Olympus manual 1 6 A T time lag Laser Class Class 3B If the electricity supply does not co...

Страница 37: ...ith at least 1 GB free space as an NTFS drive NOTE We have locked the system into using an NTFS drive because the alternative FAT32 file system doesn t provide enough protection at a folder and file l...

Страница 38: ...at installation of the software can be undertaken and that the necessary system registry updates that form part of the installation process are successfully completed Administrators have the capabilit...

Страница 39: ...points 1 for the microscope 1 for the spectrometer 1 for the stage control box and 2 for the PC The workbench must be flat and level The workbench where the spectrometer and PC are to be located shou...

Страница 40: ...The microscope module weighs approximately 20 kg Laboratory benching must be strong enough to support the combined weight of the instrument and any ancillary equipment without warping or wobbling The...

Страница 41: ...Installing Your RamanMicro 200...

Страница 42: ...8871 Spectrum Std for Raman Software Kit Contains the Spectrum Std Software CD LX100884 the Raman Instrument Configuration Disk LX108869 the Raman Instrument Installation Disk LX108925 and the RamanMi...

Страница 43: ...trometer and microscope modules both weigh 20 kg To avoid injury or damage to the instrument please use proper lifting techniques WARNING To lift and or move the instrument safely requires two people...

Страница 44: ...rlock must be satisfied You must connect an external door interlock to the 7 way DIN connector labeled INTERLOCK on the rear panel of the spectrometer or attach the External Interlock Override L132026...

Страница 45: ...s described in this section Fitting the Stage on the Microscope To fit the stage 5 Unpack the stage from the shipping material 6 Remove the objectives from the microscope 7 Carefully locate the stage...

Страница 46: ...k to the socket labeled JOYSTICK on the rear panel of the stage controller box Figure 16 Figure 16 Rear panel of the Stage Controller 2 Connect the cable from the socket on the right side of the stage...

Страница 47: ...sleeve over the z axis drive control on the left side of the microscope and then tighten the connections using an allen key OR Push the focus motor into the sleeve until it touches the drive control...

Страница 48: ...e illumination source in position against the rear of the illuminator module 2 Using the allen key supplied Figure 18 fasten the two screws so that the source is supported 3 Connect the black cable fr...

Страница 49: ...th the illuminator module and then tighten the grub screw Figure 19 Connecting the Raman module Connecting the Alignment Camera to the Raman Module The alignment camera module is then connected into t...

Страница 50: ...nsmits the collected Raman data back to the detector 1 Connect the excitation cable L1323331 from the Raman module to the EXC coupler on the rear panel of the spectrometer Ensure that the end of the f...

Страница 51: ...end of the fiber with the white cable marker is attached to the Raman module Make sure that the polarizing ridge on the FOP cable is aligned with the slot in the coupler casing before carefully pushin...

Страница 52: ...cords from the PC and the monitor into the mains electrical supply and then switch on 2 If you are prompted to login to Windows when you start the PC log in If your PC was provided by PerkinElmer you...

Страница 53: ...d in a safe place Replacement keys L1323503 can be obtained from PerkinElmer A laser safety officer or lab manager can control use of the instrument with these keys 2 Connect the instrument power supp...

Страница 54: ...propriate between the PC and triggered fiber optic probe are NOT connected The USB cable from your alignment camera if fitted is not connected into the USB socket in the sample compartment of the Rama...

Страница 55: ...on program does not start automatically from the Start Menu select Run The Run dialog is displayed 3 Enter d Setup exe and then click OK Replace d with the drive letter for your CD The InstallShield W...

Страница 56: ...o install 6 Select the required product s and then enter the License Number s as found on the Software License Certificate You must enter the correct license number s to proceed with the installation...

Страница 57: ...stall the Program page is displayed 8 Click Install to begin installing Spectrum The Installing Spectrum page is displayed which informs you of the status of the installation When the installation is...

Страница 58: ...ive 11 If the installation program does not start automatically from the Start Menu select Run The Run dialog is displayed 12 Enter d setup exe and then click OK Replace d with the drive letter for yo...

Страница 59: ...ur RamanMicro 200 59 13 Click Next The License Agreement page is displayed 14 Read the license and if you accept the terms select that option and then click Next The Ready to Install the Program page...

Страница 60: ...nstrument The Installing Raman Instrument page is briefly displayed During this installation the DirectX Setup page is displayed 16 Read the license and if you accept the terms click Yes The Installin...

Страница 61: ...from your alignment camera on the instrument and or the microscope is not connected into the USB socket in the sample compartment of the instrument 18 Click Start The Enter Instrument Details page is...

Страница 62: ...he Administrator User name that you use for the PerkinElmer software that is already installed on the PC The default User name and Password are administrator The Locate Instrument Configuration Files...

Страница 63: ...he instrument control USB cable and reattach it to a different USB port then you will be prompted for the drivers By default this driver is stored in C Program Files PerkinElmer ServiceIR Raman 24 Wai...

Страница 64: ...nstallation in the same way as for IDUS 26 Select Install from a list or specific location Advanced and then click Next The Please choose your search and installation options page is displayed 27 Sele...

Страница 65: ...zard closes 30 On the Install PerkinElmer Raman Instrument page click Continue The Instrument Installation Complete page is displayed 31 Click Finish The Raman Instrument Installer closes When the ins...

Страница 66: ...instrument The Found New Hardware Wizard is displayed 35 Repeat steps 25 to 29 to install the drivers for the alignment camera 36 If you have a Videology alignment camera you will need to install the...

Страница 67: ...200 67 38 Click Next The Please Plug in Your Camera Now page is displayed 39 Click Next The Your Camera is Successfully Installed page is displayed 40 Click Finish The Videology USB2 0 Camera Softwar...

Страница 68: ...ome to the InstallShield Wizard for Videology USB Viewer is displayed 42 Click Next The Choose Destination Location page is displayed 43 If required click Change and navigate to your chosen destinatio...

Страница 69: ...Installing Your RamanMicro 200 69 44 Click Install The Setup Status page is displayed When the installation is complete the InstallShield Wizard Complete page is displayed...

Страница 70: ...Raman Triggered Fiber Optic Probe leaflet L1321887 and then connect the fiber optic cables from the triggered fiber optic probe to the RamanMicro 200 as described for a standard fiber probe in Attach...

Страница 71: ...ce can fit up to 4 objectives 1 Select the highest magnification objective lens on the microscope either the 100x or 50x 2 Select the Bright Field BF switch position on the Olympus illuminator module...

Страница 72: ...n can be made in the software If the laser spot is very far from the crosshair a complete realignment of the video camera is required 1 Select the 50x objective lens on the microscope 2 Place a flat s...

Страница 73: ...from the Calibrate menu to save the new settings Aligning the Raman Coupler You should only adjust the Raman coupler if the signal throughput has decreased This may occur if you have moved the unit T...

Страница 74: ...nly needs realignment if the beam is observed to move grossly in the xy plane when moving into and out of focus If the laser path is aligned correctly then the laser spot should get larger in off focu...

Страница 75: ...t Please contact PerkinElmer for advice about removing the fiber optic cables that connect the spectrometer and microscope modules If you need to ship your instrument you must use the original supplie...

Страница 76: ...76 RamanMicro 200 Series Getting Started Guide...

Страница 77: ...Using the RamanMicro 200...

Страница 78: ...at the instrument is receiving power Laser This LED indicates the power status of the laser If the LED is illuminated then the laser is receiving power This does not mean that laser radiation is prese...

Страница 79: ...be body in any chemical or aggressive environment you must use an immersion sleeve to protect the probe body DO NOT ATTEMPT TO USE THE PROBE BODY IN SUCH AN ENVIRONMENT WITHOUT THE USE OF AN IMMERSION...

Страница 80: ...oving the FOP If it is necessary to remove the FOP for example for replacement or transportation please follow this procedure carefully 1 Ensure the attenuator cap is attached to the FOP 2 Switch off...

Страница 81: ...uid if you have purchased an immersible fiber probe sleeve Do not totally submerge the probe It is only acceptable to immerse the probe up to 1 inch from the top of the sleeve It may be useful to focu...

Страница 82: ...care taken in focusing the probe within the sleeve can result in a dramatic improvement in spectral quality Sampling Gases Although Raman scattering from gas samples is very weak the FOP can be used t...

Страница 83: ...eneral SOPs demonstrate how to collect data when your samples are on the microscope stage For SOPs that demonstrate how to collect data using a fiber optic probe please refer to Fiber Optic Probe SOPs...

Страница 84: ...ve the spectrometer module switched on but switch the PC off overnight if required 1 Shut down Spectrum software by selecting Exit from the File menu to return to the Windows operating environment 2 S...

Страница 85: ...g long working distance objectives for example Long Working Distance Objective Lens Kit L1323347 the working distance depends on the objective usually 2 10 mm Set the highest position of the stage to...

Страница 86: ...p down list 8 Focus on the sample using the video camera Use Video Preview on the Scan and Instrument Setup dialog Take care not to crush the sample 9 Use the stage to accurately position the sample i...

Страница 87: ...otorized stage WARNING When you are using a motorized stage do not place your fingers between the moving and fixed parts of the stage The motors driving the stage are powerful and do not stall easily...

Страница 88: ...n time in the range 0 1 10 seconds The sample may not be positioned and aligned correctly for optimized data collection The spectral data can be optimized by manipulation of the sampling accessory mot...

Страница 89: ...e options 7 On the Hardware tab select the appropriate objective lens 8 If you wish these parameters to be stored as a new set of experimental setup default parameters select Save As from the File men...

Страница 90: ...mber of scans and a minimum of 1 Background Handling Renew background at start of each sample Experiment page Advanced tab Yes Sampling Area Experiment page Sampling tab Select the number and style of...

Страница 91: ...in which the scan data will be stored NOTE If you choose to save spectra to a folder other than the default one specified for your instrument the data will not be displayed automatically To view the s...

Страница 92: ...d so as to achieve the best spectrum Laser light will be emitted from the fiber optic during data collection and when the software is running in Spectral Preview mode SOP 9 Sample Positioning when usi...

Страница 93: ...0 1 10 seconds The sample may now be positioned and aligned correctly for optimized data collection The spectral data can be optimized by adjusting the distance between the fiber optic probe and the s...

Страница 94: ...r of the instrument or accessory or if there have been appreciable temperature variations in the local environment Backgrounds are always collected during a calibration procedure The background measur...

Страница 95: ...the instrument has been brought back within specification If the wavelength calibration verification fails because the spectral intensity is low then this may also be due to the calibration sample hol...

Страница 96: ...m the Beam path drop down list 5 Click Spectral Preview 6 Focus on a sample of polystyrene L1181228 7 When the polystyrene sample is optimally focused close the Scan and Instrument Setup dialog by cli...

Страница 97: ...0 Wavelength calibration should only be performed if the instrument has failed a wavelength calibration verification 1 Ensure the RamanMicro 200 is switched on If not follow SOP 1 2 Attach the externa...

Страница 98: ...frequency calibration should only be performed if the instrument has failed a wavelength calibration verification 1 Ensure the RamanMicro 200 is switched on by following SOP 1 2 Select the 50x object...

Страница 99: ...be purchased from PerkinElmer L1321831 Your instrument will be intensity calibrated prior to shipping and during installation 1 Ensure the RamanMicro 200 is switched on if not follow SOP 1 2 Select t...

Страница 100: ...e fiber optic probe has a lens cap in place during intensity calibration of the objectives Summary The only calibration procedure that needs to be performed on a regular basis is the wavelength calibr...

Страница 101: ...ve lenses Sampling Accessories Part Number Description L1323514 Quartz microscope slide 25 75 1 mm L1860335 Tablet holder kit for motorized stage L1860336 Powder holder kit for motorized stage L132167...

Страница 102: ...working temperature 200 C maximum working pressure 1500 psi 5 M cabling L1320012 Raman probe max suitable for non contact and liquid immersion Maximum working temperature 500 C maximum working pressu...

Страница 103: ...Maintenance and Troubleshooting...

Страница 104: ...abrasive or solvent based cleaning materials Always perform a patch test on an inconspicuous area before you clean the entire instrument Avoid spilling liquid into the instrument or accessories Clean...

Страница 105: ...ower supply unit 2 Disconnect the line power cord 3 Remove the 4 screws holding the top cover in position and then lift off the cover Figure 35 Removing the top cover of the spectrometer module 4 Uncl...

Страница 106: ...neglect misuse or as a result of service or modification by anyone other than PerkinElmer will invalidate the warranty Maintenance and Service of the Fiber Optic Probe The FOP is designed to be maint...

Страница 107: ...e stage controller disconnect it from the power supply and remove the mains cord 2 Insert a screwdriver into the slot at the side of the fuse drawer and pull out and flip to one side over the mains in...

Страница 108: ...use a USB 2 0 power hub Problem The software installation stops during the Philips VCheck step Description In the VCheck dialog that is displayed while the software performs a number of checks on your...

Страница 109: ...Hardware Wizard page is displayed NOTE The Found New Hardware Wizard page may inform you that it is helping you install software for USB Device In this case continue with the installation in the same...

Страница 110: ...es Problem A motorized sample accessory does not go to the correct position when asked Description This means that for some reason the motorized stage is jammed This typically happens if the sample ho...

Страница 111: ...for some reason it stops working that is mouse clicks have no effect Solution Hold down the CTRL ALT DELETE keyboard buttons to display the Windows Security dialog Open Task Manager and then click th...

Страница 112: ...d is displayed Solution Close and open the software Problem Need to upgrade to a new version of Spectrum software Description You must uninstall the current version of Spectrum before installing a new...

Страница 113: ...ntamination The customer s responsible body is required to follow the Equipment Decontamination Procedure and complete the Certificate of Decontamination These documents are available on the PerkinElm...

Страница 114: ...is the most effective means of protecting the customer s environment The requirements for waste collection reuse recycling and recovery programs are set by the regulatory authority in your location Co...

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