PerkinElmer Raman Micro 200 Series, Руководство по началу работы

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Using the RamanMicro 200 . 87
14. Select the Dark Field (DF) switch position on the Olympus illuminator module.
You are now ready to acquire data. For more information see SOP-6 and SOP-7.
Remember, there is no “best” objective. High-magnification objective lenses give the most
efficient Raman collection from thin samples, like films or wafers, but low magnification
objectives are better for macro samples such as tablets, bulk plastics and tissue samples.
NOTE: Raman spectra are measured in the visible and near-infrared spectrum. Room lights
and sunlight will contaminate your spectra. Take care not to misinterpret room lights
or sunlight features as Raman features.
SOP-5: Sample Positioning using a Motorized Sample
Stage
NOTE: The RamanMicro 200 system can be purchased with either a manual stage or with a
motorized stage.
WARNING
When you are using a motorized stage, do not place your fingers
between the moving and fixed parts of the stage. The motors driving
the stage are powerful and do not stall easily.
You can use the Spectral Preview button in the Scan and Instrument Setup dialog to help
you position the sample for optimum spectral collection efficiency.
Sample positioning and alignment in preview mode allows you to position and focus on a
specific area and to collect spectral data from that location. It can also be used to enhance
the signal-to-noise ratio of spectral data. The data displayed on screen during a spectral
preview cannot be saved. To save data you must collect spectra (SOP-7).
1. Follow SOP-1 to turn the instrument on.
2. Load the appropriate sample holder onto the sample stage.
3. Select Setup from the Instrument menu in Spectrum.
The Scan and Instrument Setup dialog is displayed.
4. Click Spectral
Preview.
The RamanMicro 200 will start collecting data in the Spectral Preview window.