Optics11 Life PAVONE, Руководство пользователя

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Figure 41: Example of the safe areas for different wellplates.
Follow these steps:
1. Mount two same wellplates or custom plates on Pavone , open the “Wellplates” menu from the
main window and select the “calibrate” option (see Figure 42 -a). Make sure that the probe is
aligned with the objective (“Find Probe” function ) and then press “continue”.
2. Press the “Add” button and from the right menu on the right side select the “Wellplate” if you
want to calibrate a wellplate or “Custom” if you want to calibrate a custom plate (e.g. glass slide
or a petri dish holder) (see Figure 42 -b). In following the steps for calibrating a wellplate are
described. The same steps should be performed for calibrating the custom plates.
3. In the “General” menu, choose a “Name” a nd select the type of the “Plate” (see Figure 42 -c).
4. The calibration process should be done for four wells i.e. top-left corner and bottom-right corner
wells of wellplate in slot 1 and slot 2. These four wells can be recognized by
red squares (showing
the safe area of the well) (see Figure 42 -d).
5. Select the first well from the “Plate” menu which is well A1 from the wellplate slot 1 (“Well 1A1”).
6. Now calibrate the “Top left Position” and “Bottom right Position” of this specific well by moving
the stage in “X” and “Y” direction and the probe in “Z” direction
to see that the probe can enter
safely the well. The step size can be set in “Travel ( µm) ”. You can move XY stages by clicking on
the blue arrows on the bottom side of the current window (see Figure 42 -d). First, move the stage
to a place where the probe can safely enter the well in the “Top left position”. Then by choosing
small steps sizes (“Travel ( µm) ”) , slowly move the Z-stage down with the probe and enter the
well. If the probe can safely and freely (without collision with the well edges) enter the well, you
can press the “ Save Position ” . By this, the current “X” and “Y” coordinates of the stage will be
saved as a safe “Top left position”. In the lower menu, repeat the same procedure for the “Bottom
right Position”. In case of any doubt, you can press the “Move to point” option and
double-check
the “Top left position” and “Bottom right position” before proceeding to the calibration of the
next well. Note that the movement of the stage and probe during the calibration process can
immediately be terminated by pressing the “ Stop ” button (see Figure 42 -d).
7. Proceed and select the next well from the “Plate” menu.
8. Repeat step 6, and calibrate the “Top left position” and “Bottom right position” for the selected
well.
9. Complete the calibration process for all four wells and press “Save” (see Figure 42 -d).
10. The calibration process is completed meaning that the calibrated wellplate can be selected from
the left-
side menu in the “Wellplates” window for future experiments.