Optics11 Life PAVONE, Руководство пользователя

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Short description of main window controls (see Figure 8 and Figure 9 ):
1. Top window selection:
- Find Probe – allows calibration of probe tip position in respect to the camera image.
- Wellplates – allows moving to selected well in the plate.
- Calibration – calibration of the probe.
- Camera – camera settings and automatic image saving options.
- Options – settings for Find Surface and fitting of data.
- Config. exp. – set up indentation protocol and other steps.
- Maintenance – additional settings.
2. Probe stage controls:
- Single arrow – controls the step size of the probe stage and allows to move it up and down.
- Double arrow (Z up) – moves up the probe stage to 0 position.
- Incr. (µm) – step size.
- Set transit height – saves current probe stage position Z to “Transit( µ m)”.
- Go to transit height – moves down the probe stage to the selected height in “Transit( µ m)”.
3. Stage location:
- X(µm) – X stage coordinate.
- Y(µm) – Y stage coordinate.
- Z(µm) – probe stage coordinate.
- O(µm) – objective stage coordinate.
- “plate 1, A4” – current probe position within wellplate.
- Piezo (µm) – current position of the piezo together with the bar.
4. Microscopy:
- Bright field – turns on bright-field LED.
- Phase one – turns on phase-contrast LED suited for x20 objective.
- Phase two – turns on phase-contrast LED suited for x40 objective.
- LED intensity – adjusts LED intensity.
5. Temperature control:
- ON/OFF – turn on and off the temperature control.
- Set point ( °C ) – set temperature from 15 to 50°C.
- Air temp. ( °C ) – air temperature measured at the sensor (inside the frame).
- Humidity (%) – relative humidity.
6. Objective control:
- Auto cam adjust – automatically adjusts camera settings in “Camera” based on the current
image.
- Single arrow – controls the step size of the objective stage and allows to move it up and down.
- Double arrow (objective down) – moves down the objective stage to 0 position.
- Incr. (µm) – step size.
- Set focal plane – saves current objective stage position Z to “Focal plane( µ m)”.
- Go to focal plane – moves up the objective stage to the selected height in “Focal plane ( µ m)”.
7. Run experiment:
- Save image – saves the current camera image.
- Reset – resets all the hardware.
- Home stages – moves all stages to the initial position.