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Figure 14: Probe calibration menu.
2. Move the probe to the calibration well using ‘ Wellplates ’ window.
3. Make sure that the probe is prewetted.
4. Now you should move down the probe in “Incr. ( µ m)” steps of 5000 µm until the probe is clearly
below the liquid level “ Z(µm) ” ~15 000 µm. If needed, fill the well with more medium so that the
probe is clearly submerged but not too close to the bottom of the well or too close to the surface
of the liquid.
5. Wait a few minutes so that the probe can adjust to the new environment. When performing
experiments with temperature control, the calibration should be accomplished at the same
temperature as the measurement temperature. This is because probe is sensitive to temperature
variations.
6. Click the “ Scan OP1550 Wavelength ” button once. The interferometer screen will now show a
progress bar. The live signal window on the screen show oscillations, see Figure 15 -b (zoom in if
needed). Wait until that is finished and check if no error (see Figure 15 -d) is reported on the
interferometer screen or software. You can also check the final result of the “Wavelength scan”
in the corresponding interferometer window 15-c.