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Figure 21: Probe tip and sample in focus.
3.3 Moving within the well
3.3.1 Stage movement
The X and Y stages allow one to move the sample stages within the well. The ‘XY stage travel ( µ m)’ field
in the bottom-right of the camera feed allows the user to set a displacement (in µm) which can be used
by pressing the arrows in the camera feed window. When the probe is in the well and the edge of the
well is reached, stages won’t move and indicate an error at the bottom right corner. This is called a safety
zone which can be calibrated in the wellplate calibration window. Read Section 4.1 about wellplate
calibration.
3.3.2 Move to point
The X-Y stage can also be moved using the camera: after clicking anywhere on the camera feed window,
the green dot will appear and its coordinates will be recorded in
“ X(µm) ” and “ Y(µm) ” (see Figure 22 ).
Then, by clicking ‘Move to point’ in the ‘ Stage location ’ tab , the selected location will be brought under
the center of the probe. The “find probe” position of paragraph 2.5 and objective pixel size which is set
in “ Camera ” are used in this procedure. You can also choose the speed at which the stages will reach the
selected point at “ Speed (µm/s) ” .
Caution: if “ move to point ” is not precise, check camera alignment (Section 4.2), pixel
size in “Camera” settings and “find probe” position.
Figure 22: Move to point function settings.