8
(Controls Used)
(Page)
TRANSMITTED LIGHT BRIGHTFIELD
OBSERVATION PROCEDURE
}The following flow shows the operating procedure for the transmitted light brightfield observation which is the basic obser-
vation method of this microscope. The operating procedures for DIC observation, fluorescence DIC observation and IR DIC
observation will be described separately in Chapter 5, “OTHER OBSERVATION METHODS” on page 19.
Set the main switch to “
I
” (ON).
@Main switch
(P. 10)
²Lamp ON-OFF switch
(P. 10)
(Only when the hand switch is used)
Disengage the filter from the light
path.
³Filter turret
(P. 10)
Select the binocular light path.
|Light path selector knob
(P. 15)
Place the specimen on the stage.
ƒX-axis and Y-axis knobs
(P. 12)
Engage a low-magnification objective in the
light path.
…Swing lever
(P. 13)
Bring the specimen in focus.
†Coarse/fine adjustment knobs
(P. 11)
Adjust the brightness.
‡Light intensity control knob
(P. 10)
Adjust the interpupillary distance.
Adjust the diopter.
Adjust the light axis.
ŠBinocular tube
(P. 14)
‰Diopter adjustment ring
(P. 14)
‹Condenser height adjustment knob
(P. 16)
ŒCondenser centering knob
(P. 16)
Adjust the aperture iris and field iris diaphragms.
™Aperture iris diaphragm lever
(P. 17)
šField iris diaphragm ring
(P. 16)
Engage the objective to be used in the light
path and bring the specimen in focus.
…Swing lever
(P. 13)
†Coarse/fine adjustment knobs
(P. 11)
Engage the required filters.
³Filter turret
(P. 10)
Adjust the brightness.
‡Light intensity control knob
(P. 10)
Start observation.
Tip for microscope operation
In patch-clamp testing, switch the microscope controls cautiously and gently so that the patch
electrodes do not slip off.
