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BX51WI
3
DIC Observation Using IR
Since IR light is harmful to your eyes, use the monitor observation whenever possible even in adjustments.
1. First, perform adjustments for DIC observation without using IR.
}Do not mount the IR filter and see Section 5-1, “Differential Interference Contrast Observation” on page 19.
Important
· Focus the field iris diaphragm image (page 16).
Be sure to perform this adjustment accurately because it determines the visual performance
using the IR light.
· With DIC observation using IR, do not stop down the aperture iris diaphragm lever @ but leave
the diaphragm open.
As the contrast can be enhanced using the video enhancement function of the CCD controller,
the diaphragm should be left open in this adjustment so that the optical performance can be
manifested fully.
2. Then engage the IR filter (32BP775 to 32IR900) in the light path by pushing in the filter slider.
3. While observing the monitor, perform DIC observation using IR.
a) Turn the condenser turret to select the DIC prism matching the objective to be used (except the WI-DICD).
b) Turn the revolving nosepiece to engage the objective to be used in the light path.
# Penetration of air bubbles inside the front lens of objective will deteriorate the view. To prevent this by removing
the bubbles, turn the revolving nosepiece slightly to move the immersed objective to the left and right for a few
times.
c) Bring the specimen into focus by moving the objective up and down using the coarse and fine adjustment knobs.
4. Turning the 32POIR polarizer varies the density of the background. Set the polarizer to obtain optimum contrast for the
specimen.
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