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5-4 Macro Reflected Light Fluorescence Observation
}The macro reflected light fluorescence observation makes possible bright, low-magnification fluorescence observation by
combining low-magnification fluorescence mirror units and fluorescence objective.
1
Introduction
1. For the low-magnification fluorescence observation, use a low-magnification fluorescence mirror units.
The increased observation beam diameter of the fluorescence mirror units brightens the fluorescence by about 25%.
However, due to the large size of the fluorescence mirror units, they can be mounted only in every other position when the
BX-URA2 or BX-RFA reflected fluorescence illuminator is used (a total of 3 units can be mounted on each illuminator).
2. When performing transmitted light brightfield observation using a low-magnification fluorescence objective (2X or 4X),
also use the U-SC3 or U-UCD8 swinging condenser. If other condenser is combined, it will not be possible to illuminate
the entire field of view.
3. During low-magnification fluorescence observation, objective switching or stage movement, be careful so that the UIS2
(UIS) objective does not interfere with the specimen or culture container.
4. The low-magnification fluorescence objectives have been designed to manifest performances with no-covered dry speci-
mens to specimens located 5 mm below water surface level.
As a result, with water immersed specimens, the focused positions of these objectives are different from UIS2 (UIS)
objectives.
5. To enable macro reflected light fluorescence observation, the following modules should be replaced.
Low-magnification fluorescence
mirror units
U-MGFP/XL
U-MGFPA/XL
U-MF/XL
Sliding revolving nosepiece
U-SLRE
Low-magnification fluorescence objective
XLFluor2X/340
XLFluor4X/340
Water immersion cap for XL objective
XL-CAP
Condenser
U-SC3
U-UCD8
UIS2 objective
UMPlanFLN-W series
LUMPlanFLN-W series
LUMFLN60XW
UPlanSApo10X to 40X
UPlanFLN10X to 40X only
