29
2.
By pressing
Calibrate
(see pointer 2 in in Figure 34 b) UNO-CONTROLLER will start a routine to calibrate
its parameters using the T Sensor (see paragraph 8.2.5.1).
Calibrate UNO-CONTROLLER if you Room Temperature differs by more than ±1°C from 23°C.
8.2.1.2
Sample Control mode
To select the Sample Control mode, press on the Sample icon (see pointer 1 in Figure 35 b) and press on
Save
to confirm (see pointer 2 in Figure 33).
a
b
1
2
Figure 35. Settings
–
Control Mode
–
Sample Control mode (a
–
b).
The Sample Control mode enables the direct monitoring and the active control of sample temperature.
Moreover, the Sample Control mode ensures that the temperature sample differs by not more than 0.1°C from the
temperature set point regardless from the Room Temperature
’s
variations. The disadvantage of this control is that
it requires manual operation for the correct placement of the T sensor in a reference well.
In Sample Control mode, while running the experiment, the T Sensor needs to be placed into a dish (Petri
dish, Chambered Glass Slide, etc.) or a well (Reference Well) adjacent to your sample position.
Tip
►
Check if your Okolab H301 incubating chamber provides the Reference Well.
If you use a dish, secure the T Sensor to the bottom of the dish with commercial adhesive tape as shown
in Figure 36 a. Do not cover the tip of the T-Sensor. Fill the dish or the Reference Well with oil or distilled water.
Make sure the tip of the T Sensor is immersed in the liquid.
