18
4.
Connect the blue rigid end of TUBE-C to the
“Air + CO2 OUT”
push to fit connector of on the rear panel of
2GF-MIXER. Connect the silicon end of TUBE-C to the Gas Inlet gas connector of the Humidifier (see Figure
18). TUBE-C is equipped with a PTFE filter in the middle. Make sure to install the TUBE-C in the direction
of the gas flow indicated by the labels on both ends of TUBE-C and on both sides of the PTFE filter.
Tip
►
Replace the PTFE filter of TUBE-B and TUBE-C each 2-3 months of continuous operation or if you see
water in the inner part of the filter.
Do not use TUBE-B or TUBE-C without the PTFE filter.
TUBE-C
FL
OW
D
IR
EC
T
ION
FL
OW
D
IR
EC
T
ION
Humidifier
2GF-MIXER
Figure 18. UNO-T-H-CO2
–
TUBE-C connection.
Tip
►
Make sure to push the tubes all the way into the connectors thus avoiding any gas leak.
7.3.2.1
How to use 2GF-MIXER
The 2GF-MIXER generates a CO2/air mixture with an adjustable CO2 concentration in the range 0-15%.
The desired CO2 concentration is obtained by regulating the air and CO2 flow rate, in the range 0.1
–
1 l/min and
0.013
–
0.13 l/min respectively, using the two floating ball flow meters.
Do not use the 2GF-MIXER as closing-opening valves. Once regulated the flow rate (at inlet CO2
pressure of 1barg), use the 2GF-MIXER carefully only for fine regulations.
Follow the instructions below:
1.
Open the gas valves about 15 minutes before inserting the sample in the H301 Okolab incubating chamber.
2.
Table 2 reports the CO2 and air flow rates to be set in order to obtain a concentration of CO2 equal to 5%
at the 2GF-MIXER outlet.
“
Target Gas Percentage
”
indicates the desired outlet gas percentage that the
operator wants during the experiment.
“
Gas flow rate
”
, in litre per minute [l/min], are the values of gas
flow rate to be set on the graduated scale on the front panel of 2GF-MIXER.
“Total” column reports the
measured the total gas flow rate at the 2GF-MIXER outlet.