CHAPTER 7
86
Starting Scans Using An Internet Browser
For low resolution scans, make the scan area about 1 cm larger than
the membrane or gel on all four sides. For a 5 cm x 5 cm membrane,
set the scan
Width
and
Height
to 7 cm and set the Origin to 0,0. The
membrane would then be placed at the 1 cm x 1 cm position on the
scan surface.
Focus Offset
for scans of membranes should be zero. For gels,
Focus
Offset
should be half the gel thickness in millimeters. A focus offset
of 3.0 mm is usually accurate for standard microplates. The
maximum allowable focus offset is 4 mm (i.e., 8 mm gels).
Comments:
Comments can be used to identify the scan. In the
Odyssey
®
software, comments can be included in reports.
Starting a Scan
Before starting a scan, an estimate of the scan time can be calculated
by clicking the
Calculate Scan Time
button at the bottom of the Scan
Setup window.
5)
Click
Configure
to send the scan parameters to the Odyssey
Imager.
After the Odyssey Imager is configured, the Scan Console window is
displayed.
The scan is not actually started until the
Start
button is clicked, but
the Odyssey Imager is reserved for the user who initiated the scan.
Other users on the network will not be able to start scans until the
