21
•
Protect plates from light before imaging to ensure highest sensitivity. When
storing plates after imaging, protect plates from light at room temperature.
•
A
Focus Offset
of 3.0 mm should be entered in the scan parameters
(Chapter 2,
Odyssey® User Guide
) when using the plates specified above.
Plates other than those recommended above may require lower or higher
focus offsets for optimal resolution and detection. If alternative plates are
used, an initial optimization scan will be necessary. Scan a plate
containing experimental and control samples at 0.5, 1.0, 2.0, 3.0, and 4.0
mm focus offsets. Use the same intensity settings for each scan. After
reviewing the collected scans, use the focus offset with the highest signal
to noise as the focus offset for experiments with the alternate plates.
•
The
Intensity
scan parameter for both 700 and 800 nm channels should be
set to 5 for initial scanning. If the image signal is saturated or too high, re-
scan using a lower intensity setting (i.e. 2.5). Likewise, If the image signal
is too low, re-scan using a higher intensity setting (i.e. 7.5).
•
For satisfactory images with minimal scan time, the
Quality
scan
parameter should be medium, with
Resolution
set to 169 µm. Higher scan
quality and resolution may be used, but scan time will increase.
Using the MousePOD Accessory
Figure 3-1
. The MousePOD Accessory.
The optional Odyssey
MousePOD
®
Accessory fits
over the Odyssey scanning
surface for
in vivo
imaging of
up to three mice in a temper-
ature-controlled enclosure.
Operation of the MousePOD
Accessory is described in the
Odyssey
In vivo
Imaging
Guide available at:
http://biosupport.licor.com
