ELC-01MX User Manual
version 2.6
page 23
Figure 10: headstage connections, A: differential measurement, B: single-ended measurement
7.2 Extracellular Voltage Measurement
Extracellular measurements are usually done in a loose-patch configuration or with special
metal microelectrodes. Recordings with extracellular metal electrodes is simple. The electrode
is advanced in the region where the recordings will be made using a micromanipulator and the
signals are filtered and amplified (see chapter 5 in Lalley et al., 1999 for details). For loose
patch recording the procedure is the following (Barbour & Isope, 2000, Nunemaker et al,
2003):
❏
Approach the cell in VC mode and apply square voltage pulses to the electrode.
❏
Contact the cell and establish the loose-patch.
❏
Set the MODE OF OPERATION switch (#23, Figure 5) to OFF.
❏
Set the required amplification of the POTENTIAL OUTPUT using #20, Figure 5.
❏
Select DC or filtered AC coupling with switch #19, Figure 5.
❏
If needed apply the LP filter (3 kHz) to the output signal (see #11, Figure 5).
