Operating Procedures
0112-0127 B
91
•
Time Point:
(Minimum Time to 9999 seconds) This setting
determines the time after the start of the reading when the fluid
is scheduled to begin being dispensed. Your time point will
eventually be your baseline time. This is not the time interval
between transfers. In Endpoint and Kinetic reads, Time Point
reflects the time at which the first dispense occurs. The Endpoint
or Kinetic read begins after all transfers are made.
•
Minimum Time:
This information line automatically displays the
minimum time required before a pipetting event can occur. This
minimum time value is cumulative, not an interval between
pipetting events. The value is the minimum number of seconds
of elapsed time from the beginning of the read. It takes into
account the mechanical speed of the pipette head and the time
needed to load and unload tips, aspirate and dispense fluids,
trituration, and Automix.
The minimum time for the second pipetting event depends on
when the first pipetting event occurred. The calculation for the
second event starts at the end of the first event and adds the
total time necessary to load pipette tips, aspirate new fluids from
the compound plate, and dispense them into the assay plate.
Possible Problems
Time point calculations are based on the number of wells read, the tip
column and compound column used during transfer. If you select time
points that are not long enough (incompatible with the selected volumes
and transfer speed), the system notifies you that the time point needs to
be increased.
If you select transfer volumes that add up to more than the maximum
that can be accommodated by the assay plate, an overflow warning
appears at the bottom of the settings screen indicating you have
exceeded the volume limits of the read plate.
Pay attention to the minimum time value displayed under the time point
field for each transfer, as the minimum time values are different
depending on which transfer you are configuring.
The Compound Transfer setting works in conjunction with the Compound
& Tip Columns setting. Until well assignments are made in the
Compounds & Tip Columns setting, the Compound Transfer setting
displays
No targets assigned
.
Note:
The Time Point cannot be smaller than the Minimum
Time identified by the software for each transfer.
FlexStation_manual.book Page 91 Tuesday, June 25, 2013 10:22 AM
