FlexStation 3 Benchtop Multi-Mode Microplate Reader User Guide
104
0112-0127 B
Emission Cutoff Filter
The emission cutoff filters assist in reducing background. Sources of
background include stray excitation light and native fluorescence of
microplate materials, sample constituents, and solvents (including
water). The default setting allows the instrument and the SoftMax Pro
Software to determine which cutoff filter should be used in Endpoint and
Kinetic reads. For default settings, see
spectral scan read default uses no cutoff filter.
Readings Per Well
The number of readings per well may vary between 1 (used for a quick
estimate) and 100 (for very precise measurements). The default number
of readings per well varies with the read mode. For fluorescence, the
default is 6, and for luminescence, the default is 30.
PMT Voltage
The voltage of the photomultiplier tube can be set to low (for higher
concentration samples), medium, or high (for lower concentration
samples) in all read modes. In Endpoint and Spectrum reads, there is an
additional setting, automatic, in which the instrument automatically
adjusts the PMT voltage for varying concentrations of sample in the
plate.
Temperature Control
The reading chamber of the FlexStation 3 instrument is isothermal at
ambient as well as at elevated temperatures. The temperature in the
reading chamber can be adjusted from 2°C above ambient to 45°C.
Note:
Assay optimization requires the use of a computer and SoftMax
Pro Software.
FlexStation_manual.book Page 104 Tuesday, June 25, 2013 10:22 AM
