FlexStation 3 Benchtop Multi-Mode Microplate Reader User Guide
38
0112-0127 B
Assay Read Types
The instrument operates in three integrated fluidics/read (Flex, Endpoint,
and Kinetic) read and two read types. The Table 1-1 on page 39 compares
the types of operation and features that are available for the different
read types.
Flex Reads
The fluidics module is designed to aspirate fluids from a compound
source plate and dispense them into an assay plate. Fluid transfer is
made possible with an 8-channel or 16-channel pipettor that is fully
automated, including changing the tips from a tip rack.
In Flex reads, one to eight or one to sixteen wells in one column of the
assay plate are read repeatedly for a selected total experimental time. At
a preselected point or points during that time sequence, the pipettor can
transfer up to three reagents from the compound plate to the assay
plate. The instrument continues to read at the preselected time intervals
before and after each fluid transfer. After completion of reading the
column (or partial column) for a preselected time, the instrument can
repeat this cycle with other columns. All the data is collected in one data
file represented as a 96-well or 384-well microplate.
For example, an experiment with a two-minute run time accommodates
a 96-well microplate in about 24 minutes.
Run time × Number of columns = Plate time
2 minutes × 12 columns = 24 minutes
Note:
This user guide describes instrument behavior for Flex read type
primarily. For instructions on other read types, see the SoftMax Pro
user guide.
FlexStation_manual.book Page 38 Tuesday, June 25, 2013 10:22 AM
