FlexStation 3 Benchtop Multi-Mode Microplate Reader User Guide
90
0112-0127 B
Transfer Settings
•
Pipette Height:
(1
μ
L to 999
μ
L) This setting determines the
volume of fluid (in microliters, measured from the bottom of the
assay plate microplate well) above which the tip of the pipette
will remain during the dispensing portion of the transfer event.
This setting helps ensure that the tip of the pipette is below the
surface of the liquid at the end of the transfer, minimizing the
possibility that undispensed drops remain on the tips.
•
Rate
: (1 to 8) This setting determines the rate at which the fluid
is dispensed into the well of the assay microplate. In a 96-well
microplate, a setting of 1 is equal to 26
μ
L per second and each
subsequent number increases in increments of 26 microliters.
Therefore, a setting of 2 is equal to 52
μ
L per second. A setting of
3 or 4 can help minimize cell damage or dislodgment. In 384-well
microplates, the speed is set to 6
μ
L per second and increases in
increments of 6
μ
L.
•
Volume:
During Flex mode, this setting determines the volume
of material to be dispensed from the source to each individual
well chosen to receive that transfer.
For a 96-well microplate, the range is 1
μ
L to 200
μ
L.
For a 384-well microplate, the range is 1
μ
L to 30
μ
L.
Note:
As you configure subsequent transfers you must calculate
the amount of fluid added and set the pipette height
accordingly.
Note:
For non-contact dispensing, use a rate of 8 to ensure all
liquid is dispensed from pipette tip.
Note:
Keep in mind the maximum total volume each well can
hold as you accumulate volumes with multiple transfers.
FlexStation_manual.book Page 90 Tuesday, June 25, 2013 10:22 AM
