Milestone PrestoCHILL, Руководство оператора

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PrestoCHILL MM138-003
– Operator Manual
58
11) Put the extractor over the ring and push down in order to move up the block and outside the ring.
If block extraction is difficult, warm the base of the block
with a gloved hand. This will soften residual MCC that is
formed under the ring.
12) Trim and cut in the cryostat.
A.6. Freezing of high sample in vertical position
This procedure is for the freezing of samples long up to 2.5cm that must be frozen in vertical position. For
example, in case of rat or mouse brain that must be cut in frontal (coronal) sections, see picture below:
Milestone suggests to freeze sample inside high ring at a temperature of -80°C. This allows a
fast enough freezing also of big samples.
1) Remove from the PrestoCHILL working area the heat extractors and the six moulds, unscrewing
them. Insert the pad and then the ring plate with the serigraphy down. Add the needed tools.
Because this type of ring is high, to use the heat extractor and close the PrestoCHILL cover
during the freezing, it is necessary to remove the handle from the heat extractor.
It works correctly enhancing the freezing of the sample.