BoneSTATION
MM093-002
– Operator Manual
40
7.2.2. Protocols for decalcification of bone marrow biopsies 5mm
MoL-DECALCIFIER 37°C:
TOTAL TIME 43 hours 30 minutes
PHASE
TEMPERATURE
TIME
PRESOAKING
R/T
3 hours
FIXATION
37°C
4 hours 30 minutes
EDTA 10%
37°C
36 hours
MoL-DECALCIFIER 50°C:
TOTAL TIME 14 hours
PHASE
TEMPERATURE
TIME
PRESOAKING
R/T
3 hours
FIXATION
50°C
2 hours
EDTA 10%
50°C
9 hours
FORMIC ACID 10% 37°C:
TOTAL TIME 12 hours
PHASE
TEMPERATURE
TIME
PRESOAKING
R/T
3 hours
FIXATION
37°C
4 hours 30 minutes
FORMIC ACID 10%
37°C
4 hours 30 minutes
FORMIC ACID 10% 50°C:
TOTAL TIME 7 hours
PHASE
TEMPERATURE
TIME
PRESOAKING
R/T
3 hours
FIXATION
50°C
2 hours
FORMIC ACID 10%
50°C
2 hours
HYDROCLORIC ACID 10% 37°C:
TOTAL TIME 9 hours
PHASE
TEMPERATURE
TIME
PRESOAKING
R/T
3 hours
FIXATION
37°C
4 hours 30 minutes
HYDROCLORIC ACID 10%
37°C
1 hour 30 minutes
NITRIC ACID 10% 37°C:
TOTAL TIME 9 hours
FASE
TEMPERATURE
TIME
PRESOAKING
R/T
3 hours
FIXATION
37°C
4 hours 30 minutes
NITRIC ACID 10%
37°C
1 hour 30 minutes
