micronit microfludics FLUIDIC CONNECT 4515, Руководство пользователя

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5 TIPS AND TRICKS FOR CONNECTION
5.1 TIGHTENING FORCE
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Please read the tips and tricks before connecting Fluidic Connect 4515.
Why is the tightening of the connections specified?
1. To create a proper sealing between the ferrule and the chip, the ferrule must be pressed
tight against the chip.
2. Once the seal is created, additional force enables a higher back pressure of the fluid, but
the additional force needed for e.g. 100 bars, is relatively small. Tightening finger tight
is estimated to be roughly 7cNm of torque.
3. Applying even more torque is unnecessary; it will only stress the chip, possibly breaking
of the chip or permanently deforming the ferrule.
The manual says that I should tighten the connection finger tight, how do I know if the connection is finger tight?
Note that over tightening can break the glass chip, especially when working with a thin-bottom
chip like the R50.332.3 or the X3550CH.3 chip. Use the following protocol to avoid breaking of
microfluidic chips.
1. To tighten finger tight, you should twist the connection with the tip of your thumb and
index finger, until you feel the ferrule pressing onto the chip (tightening resistance
increase). You should not try to get a better grip on the connection, just proceed with
step 2.
2. After finger tightening, tighten the nut with the extender tool:
another 1/4 round for Flat-Bottom connections (Teflon / Stainless Steel)
another 1/3 round for Nanoport connections (Fused Silica)
Figure 5.1. Extender tool, for 1/4 or 1/3 round additional tightening
Fluidic Connect 4515 - User Manual V1.5 15