micronit microfludics FLUIDIC CONNECT 4515, Руководство пользователя

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How many times can I use a ferrule?
Both Nanoport and Flat-bottom ferrules can be used multiple times. There are some factors
that can reduce the lifespan of the ferrules.
When using the Flat-bottom ferrules it is possible that the ferrule are tightened over the
edge of the glass holes, which can damage the sealing area of the ferrule. After reusing the
ferrule multiple times the damage can be cause leakage and replacing the ferrule is
required. The damage should be visible to the naked eye.
The seal is created by elastic deformation of the ferrule. Too much tightening can cause
plastic deformation, reducing the ability to create a functional seal.
When using Nanoport ferrules, the ferrules are sensitive to thermal cycles. Unloaded
ferrules can withstand high temperatures up to 200°C, but when compressed to create the
seal (applying pressure and torque on the material), temperatures above 50°C are not
advised. Also long term experiments can result in chemical changes of the ferrule, reducing
its function.
I can see fluid flowing in degassing lines, is the chip broken?
The glass chips contain so-called degassing channels; these are non-functional channels
solely used for manufacturing purposes. When you see fluid in these degassing channels,
this does not mean that the chip is broken. Normally there is no water in the degassing
channels, but it is possible that during cleaning or after previous leakage, fluids flows in
these degassing channels from the sides of the chip. When fluids are visible check the fluidic
connections to see if there is no leakage from these fluidic connections.
Is it possible to place the chip holder in a bath with an elevated temperature?
The Fluidic connect has a maximum working temperature of 50-80C depending on the
connection type used. The Nanoport ferrules (supplied with Fused Silica Connection Kit) can
only be used up to 50°C when tightened. The materials used are all corrosion resistant and
can be used under water. Note that when using Nanoport ferrules, there is a chance that the
ferrules can leak due to softening (which is reversible) of the material when the initial
tightening force was insufficient to compensate for this 'loss of physical properties'. The loss
of physical properties also depends on the temperature, chemicals used and applied
tightening force (torque). With increasing time at high temperature, chemical changes
occur. These generally cause an increase in hardness, along with volume and compression
set changes.
Fluidic Connect 4515 - User Manual V1.5 14