micronit microfludics FLUIDIC CONNECT 4515, Руководство пользователя

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2.4 STEP 4: CONNECTION OF FITTINGS
2.4.1 STEP 4A - FUSED SILICA CONNECTION KIT
Now the fluidic connections can be made. Since different connection types require different
procedures, the connection procedures are described separately below. The procedure for the
Fused Silica Connection Kit is described in
Step 4A
, for the Teflon and Stainless Steel Connection
Kit the description can be found in
Step 4B
.
Note that over tightening can break the glass chip, especially when working with a thin-bottom
chip like the R50.332.3 or the X3550CH.3 chip. Use the following protocol to avoid breaking of
microfluidic chips.
Step 1. To tighten finger tight, you should twist the connection with the tip of your thumb and
index finger, until you feel the ferrule pressing onto the chip (tightening resistance
increase). You should not try to get a better grip on the connection, just proceed with
step 2.
Step 2. After finger tightening, tighten the nut with the extender tool:
- another 1/4 round for Flat-Bottom connections (Teflon / Stainless Steel)
- another 1/3 round for Nanoport connections (Fused Silica)
Step 4A is specifically for the Fused Silica Connection Kit.
1. Adjusting capillary length
The ‘Nanoport’ ferrules and nuts supplied with the Fused Silica Connection Kit enable low
dead-volume connections, using fused silica capillary tubing of 375 µ m outer diameter.
Various tools are available for cutting capillaries to the required length. The capillary can
be cut with a straight edge using a dedicated tool. A well-defined cut is important in order to
minimize the dead volume in the connection between the capillary and the chip.
Alternatively, the capillary can be cut using a sharp knife.
Figure 2.4. Fused silica Figure 2.5. Cutting the Figure 2.6. Capillary that
with a polyimide coating capillary has been cut
Fluidic Connect 4515 - User Manual V1.5 7
Silica
Buffer
Coating
ID
CT
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