4.4 Purging the ultrafiltration cell
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IC equipment: Inline Ultrafiltration 2 - push mode
Insert the ultrafiltration cell in such a way that the positioning pins of
the filtration cell holder are located in the openings of the filtration
cell provided for this purpose.
4.4
Purging the ultrafiltration cell
Every time a new filtration membrane is inserted, purge the filtration cell.
To accomplish this, rinse all capillaries with e.g. ultrapure water. The entire
filtration system must be completely connected prior to this procedure.
Rinsing the ultrafiltration cell
1 Settings in the software
￭
Immerse the sample needle in the rinsing solution.
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Switch on the peristaltic pump.
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Rinse the filtration system with ultrapure water for approx. 5 min.
2 Monitoring the rinsing process
￭
Check whether equal amounts of solution are emerging from
both feed lines to the waste container.
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Check whether all capillary connections are tight.
If liquid is escaping somewhere, then tighten the corresponding
connection or redo the connection.
3 Removing air bubbles
￭
Check whether any air bubbles remain trapped in the ultrafiltra-
tion cell.
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If air bubbles are trapped in the cell, then unscrew the PTFE capil-
laries from the filtrate outlet and from the sample outlet and wait
until the air bubbles have escaped. Afterwards, tighten the capilla-
ries to the ultrafiltration cell again.
