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3.3 Installation of the low pressure gradient
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850 Professional IC–Anion – MSM-HC – MCS – LP Gradient
3.3 Installation of the low pressure gradient
The installation of the 850 Professional IC – Anion – MSM-HC – MCS – LP
Gradient must be adjusted slightly in order to be operated as a low pres-
sure gradient system. The following installation instructions list the neces-
sary work steps in brief. At each step you will find cross-references to
more detailed installation instructions for individual components, should
you require such aids.
Installing the low pressure gradient
1 Expanding the instrument to low pressure gradient
See Chapter 3.11, Page 38
￭
Loosen the 6.1834.090 connection tubing from the output of the
eluent degasser.
￭
Dismount the coupling (21- 9 ) and pump head inlet capillary
(21- 7 ) of the high pressure pump.
￭
Use the short pressure screw to tightly screw the 6.2758.020 mix-
ing coil for low pressure gradients directly onto the input of the
high pressure pump,
(see Chapter 3.12.2, page 43) and the lon-
ger pressure screw to screw it tightly to the output of the mixing
valve
(see "Connecting mixing coil for low pressure gradients",
page 40) .
￭
Screw on the 6.1834.120 connection tubing to the output of the
eluent degasser
(see "Connecting the eluent degasser", page
37) and connect with input A of the mixing valve (see "Connect-
ing connection tubings", page 41) .
￭
Equip two 6.1834.080 eluent aspiration tubings (see "Assembling
eluent aspiration tubing", page 33) and screw tightly to the elu-
ent degasser inputs in the low pressure gradient (see "Connecting
the eluent degasser", page 37) .