Medonic CA530, Руководство пользователя

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Calibration and controls
1009en01 03-11-24 85
4. Enter the target value read from the assay sheet in the field “Target” and
press [Enter]. The analyzer will calculate the new calibration factor and dis-
play this in the field “New”.
In the example above, a RBC target value of 4.40 is entered, resulting in a
new calibration factor of +1.8%. Next time this menu is recalled, the field
“Cal% org.” will show 1.8%.
5. Scroll to the next parameter to be calibrated using the arrow keys and repeat
the above point 4.
Note:
Sample runs with (SE, DE etc.) are not included in the 5 samples displayed in
menu 5.1-5.3. This is shown in the calibration menus as =0 at the sample display.
10.5 Calibration on Pre-Diluted Capillary
Blood
Calibration for the capillary blood inlet is done in the same way as at the whole
blood inlet. Dilute the capillary blood 1:200 e.g. 25 (alternative 20) µl in 5ml dilu-
ent, 30µl into 6ml or 40µl in 8ml. Anything between these values is accepted as
long as the total volume is between 5 and 8 ml with a dilution ratio of 1:200 to
1:250 and is reproducible. Enter the calibration-menu for pre-diluted capillary
blood from menu 5.2 and set the calibration factors.
A practical method is to first calibrate the whole-blood inlet. Secondly, run a nor-
mal sample at the open tube inlet and note down the parameter values, then use
this sample to calibrate the pre-dilute-inlet of the instrument. Note that the pre-
dilute-inlet is not factory calibrated, as the setting is dependent on locally used
capillaries and dilution ratio.
10.6 Calibration of the MPA
The calibration of the Micro Pipette Adapter parameters is similar to the whole
blood and/or pre-diluted blood calibration. The recommended calibration meth-
od is to run a vein blood sample in the (calibrated) whole blood inlet and to com-
pare the results of the same blood at the MPA inlet.
Note that some discrepancies might be observed when comparing to venous
blood and Micro Pipette collected blood from a finger puncture (same patient).
This is a pre-analytical error that might occur especially if the finger puncture is
not done according to the specifications. PLTs might aggregate in case of a bad
blood collection procedure. This may also give erroneous results in the total
WBC and 3 part differential count. Typical differences between vein and finger
puncture collected samples are listed in a report available from your authorized
distributor.
Please point your browser to:
www.medonic.se/MPA and down load the movie
sequences how to operate the MPA with the Micro Lancet as supplied by Boule
to minimize such pre-analytical errors.
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